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A9253

L-Amino Acid Oxidase from Crotalus adamanteus

Type I (dried venom)

Synonym(s):

L-AAO, L-Amino acid: oxygen oxidoreductase (deaminating)

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250 mg
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$723.00

About This Item

CAS Number:
9000-89-9
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-564-0
MDL number:
MFCD00131454
EC Number:
1.4.3.2 (BRENDA, IUBMB)
Biological source:
Crotalus adamanteus

$723.00

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biological source

Crotalus adamanteus

Quality Segment

200

type

Type I (dried venom), Type I

form

powder

mol wt

~130 kDa

solubility

H2O: soluble 1.0 mg/mL, clear(lit.)

storage temp.

−20°C

General description

L-Amino acid oxidase (LAAO) is a flavoprotein with a molecular weight of 130 kDa. It consists of two different subunits of approximately 70 kDa. Each molecule of holoenzyme has two flavin adenine dinucleotide (FAD) molecules. LAAO is a glycoprotein containing about 2-5% carbohydrate, including sialic acid. optimum pH is approximately 7.5. It occurs in many snake venoms apart from microorganisms and animal tissue, especially in kidney. In the N-terminal region, it has a βαβ domain with glutamic acid residues. LAAO imparts yellow color to venom.

Application

L-Amino Acid Oxidase from Crotalus adamanteus has been used in the quantification of rid protein in imine deaminase activity.
L-amino acid oxidase (LAAO) is used to convert L-amino acids to their corresponding α-keto acids. L-amino acid oxidase, from Sigma, has been used in leucine aminopeptidase (LAP) activity assays.[1] The enzyme has been immobilized and used in an enzymatic flow-injection procedure with chemiluminescence detection for on-site determination of L-alanine.

Biochem/physiol Actions

L-Amino acid oxidase (LAAO) from Crotalus adamanteus requires Mg2+ for its activation. The N-terminal region is essential for FAD binding. The H2O2 generated by LAAO based oxidation reactions lead to prevention of platelet aggregation and induces edema and apoptosis. LAAO activity is inhibited by ethylenediaminetetraacetic acid (EDTA) and phenylmethylsulfonyl fluoride (PMSF).

Preparation Note

Dissolves in water at 1 mg/mL concentration to form a clear solution.

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L-Amino Acid Oxidase from Crotalus adamanteus Type I (dried venom)

Sigma-Aldrich

A9253

L-Amino Acid Oxidase from Crotalus adamanteus

L-Amino Acid Oxidase from Crotalus adamanteus Type IV, ≥4.0 units/mg protein, aqueous suspension

Sigma-Aldrich

A9378

L-Amino Acid Oxidase from Crotalus adamanteus

L-Amino Acid Oxidase from Crotalus atrox (Western Diamondback Rattlesnake) Type VI, dried venom

Sigma-Aldrich

A5147

L-Amino Acid Oxidase from Crotalus atrox (Western Diamondback Rattlesnake)

Fructosyl-Amino Acid Oxidase from Corynebacterium sp. recombinant, expressed in E. coli, lyophilized powder, ≥0.45 units/mg protein

Sigma-Aldrich

F7296

Fructosyl-Amino Acid Oxidase from Corynebacterium sp.

description

Type I (dried venom)

description

Type IV, ≥4.0 units/mg protein, aqueous suspension

description

Type VI, dried venom

description

recombinant, expressed in E. coli, lyophilized powder, ≥0.45 units/mg protein

biological source

Crotalus adamanteus

biological source

-

biological source

-

biological source

-

form

powder

form

aqueous suspension

form

dried venom

form

lyophilized powder

mol wt

~130 kDa

mol wt

~130 kDa

mol wt

-

mol wt

~88 kDa by electrophoresis

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

solubility

H2O: soluble 1.0 mg/mL, clear(lit.)

solubility

H2O: soluble 1.0 mg/mL, clear

solubility

-

solubility

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

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pictograms

Skull and crossbones
GHS06

signalword

Danger

Hazard Classifications

Acute Tox. 1 Inhalation - Acute Tox. 2 Dermal - Acute Tox. 2 Oral

Storage Class

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Questions

  1. What is the enzyme activity of this product?

    1 answer

    1. This activity of this product is not tested on a lot-to-lot basis. However, historical data indicate a range of 0.28 - 0.40 units per milligram.

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