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Monoclonal Anti-β-Galactosidase−Biotin Conjugate antibody produced in mouse

Name: Monoclonal Anti-β-Galactosidase−Biotin Conjugate antibody produced in mouse
Brand: SIGMA

clone GAL-13, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Monoclonal Anti-β-Galactosidase

About This Item

NACRES:

NA.46

UNSPSC Code:

12352203

Conjugate:

biotin conjugate

Clone:

GAL-13, monoclonal

Application:

Citations:

This product is discontinued. Contact Technical Servicefor assistance.

Properties

biological source

mouse

Quality Level

200

conjugate

biotin conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

GAL-13, monoclonal

form

buffered aqueous solution

species reactivity

bacteria

technique(s)

dot blot: 1:2,000

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Description

General description

Monoclonal Anti-b-Galactosidase (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. β-Galactosidase enzyme is encoded by lacZ gene of E. coli.

Immunogen

β-galactosidase from E. coli

Application

Monoclonal Anti-β-Galactosidase-Biotin Conjugate antibody produced in mouse may be used with ExtrAvidin-Peroxidase in the dot blot technique on native, purified, or crude β-galactosidase. The antibody was used to detect β-galactosidase by immunofluorescence in paraffin-embedded adult mouse kidney sections. It was used in ELISPOT assays at a dilution of 1:1000.

Biochem/physiol Actions

β-galactosidase enzyme is encoded by lacZ gene of E. coli; it acts on lactose and cleaves it to glucose and galactose. Monoclonal Anti-β-Galactosidase-Biotin Conjugate antibody binds with soluble enzyme even when bound to a surface without causing any loss of enzymatic activity. It is used as readout for promoter activity in lacZ transfected cells.

The antibody may be used for amplification in immunoenzymatic staining by preparing a β-galactosidase anti-β-galactosidase (BGABG) soluble complex. It is not recommended for immunoblotting; it does not recognize denatured or reduced β-galactosidase.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

Preparation Note

Prepared by conjugation with ε-aminocaproyl biotin.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Show Differences

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This Item	G6282	B1531	D8156
Sigma-Aldrich B0271 Monoclonal Anti-β-Galactosidase−Biotin Conjugate antibody produced in mouse	Sigma-Aldrich G6282 Monoclonal Anti-β-Galactosidase antibody produced in mouse	Sigma-Aldrich B1531 Monoclonal Anti-Phosphotyrosine−Biotin antibody produced in mouse	Sigma-Aldrich D8156 Monoclonal Anti-Digoxin antibody produced in mouse
clone GAL-13, monoclonal	clone GAL-40, monoclonal	clone PT-66, monoclonal	clone DI-22, monoclonal
conjugate biotin conjugate	conjugate unconjugated	conjugate biotin conjugate	conjugate unconjugated
biological source mouse	biological source mouse	biological source mouse	biological source mouse
species reactivity bacteria	species reactivity bacteria	species reactivity -	species reactivity -
antibody form purified immunoglobulin	antibody form ascites fluid	antibody form purified immunoglobulin	antibody form ascites fluid
technique(s) dot blot: 1:2,000	technique(s) dot blot: suitable using soluble β-galactosidase, immunocytochemistry: suitable, western blot: 1:1,000 using denatured-reduced Escherichia coli β-galactosidase	technique(s) direct ELISA: 1:50,000, dot blot: 1:32,000	technique(s) dot blot: suitable, flow cytometry: suitable, indirect ELISA: 1:10,000 using digoxin-BSA, indirect ELISA: 1:2,500 using digoxigenin-transferrin

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Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

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Articles

ELISpot Assay: Functional Cellular Immunology

ELISpot assay provides qualitative and quantitative information on immune responses, visualizing multiple secretory products from single responding cells.

Open reading frame expression vectors: a general method for antigen production in Escherichia coli using protein fusions to beta-galactosidase.

G M Weinstock et al.

Proceedings of the National Academy of Sciences of the United States of America, 80(14), 4432-4436 (1983-07-01)

We have developed an Escherichia coli plasmid vector for the identification and expression of foreign DNA segments that are open reading frames (ORFs). The 5' end of ompF, an E. coli gene encoding an abundant outer membrane protein, is used

HIV-specific cytotoxic cell frequencies measured directly ex vivo by the Lysispot assay can be higher or lower than the frequencies of IFN-gamma-secreting cells: anti-HIV cytotoxicity is not generally impaired relative to other chronic virus responses.

Jennifer E Snyder-Cappione et al.

Journal of immunology (Baltimore, Md. : 1950), 176(4), 2662-2668 (2006-02-04)

CD8(+) T cells in HIV-infected patients are believed to contribute to the containment of the virus and the delay of disease progression. However, the frequencies of HIV-specific CD8(+) T cells, as measured by IFN-gamma secretion and tetramer binding, often do

Easy identification of cDNA clones.

U Rüther et al.

The EMBO journal, 2(10), 1791-1794 (1983-01-01)

A set of six cloning vectors, pUR 278, 288, 289, 290, 291, 292 is presented. These vectors have the cloning sites, BamHI, SalI, PstI, XbaI and HindIII, in all frames at the 3' end of the lacZ gene. Insertion of

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Global Trade Item Number

SKU	GTIN
B0271-.2ML	04061837856280

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