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B0271

Monoclonal Anti-β-Galactosidase−Biotin Conjugate antibody produced in mouse

Name: Monoclonal Anti-β-Galactosidase−Biotin Conjugate antibody produced in mouse
Brand: SIGMA

clone GAL-13, purified immunoglobulin, buffered aqueous solution

Synonym(e):

Monoclonal Anti-β-Galactosidase

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Über diesen Artikel

NACRES:

NA.46

UNSPSC Code:

12352203

Conjugate:

biotin conjugate

Clone:

GAL-13, monoclonal

Application:

Citations:

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Eigenschaften

biological source

mouse

conjugate

biotin conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

GAL-13, monoclonal

form

buffered aqueous solution

species reactivity

bacteria

technique(s)

dot blot: 1:2,000

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Beschreibung

General description

Monoclonal Anti-b-Galactosidase (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. β-Galactosidase enzyme is encoded by lacZ gene of E. coli.

Immunogen

β-galactosidase from E. coli

Application

Monoclonal Anti-β-Galactosidase-Biotin Conjugate antibody produced in mouse may be used with ExtrAvidin-Peroxidase in the dot blot technique on native, purified, or crude β-galactosidase. The antibody was used to detect β-galactosidase by immunofluorescence in paraffin-embedded adult mouse kidney sections. It was used in ELISPOT assays at a dilution of 1:1000.

Biochem/physiol Actions

β-galactosidase enzyme is encoded by lacZ gene of E. coli; it acts on lactose and cleaves it to glucose and galactose. Monoclonal Anti-β-Galactosidase-Biotin Conjugate antibody binds with soluble enzyme even when bound to a surface without causing any loss of enzymatic activity. It is used as readout for promoter activity in lacZ transfected cells.

The antibody may be used for amplification in immunoenzymatic staining by preparing a β-galactosidase anti-β-galactosidase (BGABG) soluble complex. It is not recommended for immunoblotting; it does not recognize denatured or reduced β-galactosidase.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

Preparation Note

Prepared by conjugation with ε-aminocaproyl biotin.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Dieser Artikel	SAB4200805	WH0002720M1	B0287
Sigma-Aldrich B0271 Monoclonal Anti-β-Galactosidase−Biotin Conjugate antibody produced in mouse	Sigma-Aldrich SAB4200805 Anti-β-Galactosidase antibody, Mouse monoclonal	Sigma-Aldrich WH0002720M1 Monoclonal Anti-GLB1 antibody produced in mouse	Sigma-Aldrich B0287 Monoclonal Anti-FITC−Biotin antibody produced in mouse
biological source mouse	biological source mouse	biological source mouse	biological source mouse
species reactivity bacteria	species reactivity E. coli	species reactivity human	species reactivity -
antibody form purified immunoglobulin	antibody form purified from hybridoma cell culture	antibody form purified immunoglobulin	antibody form purified immunoglobulin
conjugate biotin conjugate	conjugate -	conjugate unconjugated	conjugate biotin conjugate
clone GAL-13, monoclonal	clone GAL-13, monoclonal	clone 6E7, monoclonal	clone FL-D6, monoclonal
isotype IgG1	isotype IgG1	isotype IgG2aκ	isotype IgG1

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Lagerklasse

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c