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Monoclonal Anti-Bacterial Alkaline Phosphatase (BAP, PhoA) antibody produced in mouse

Name: Monoclonal Anti-Bacterial Alkaline Phosphatase (BAP, PhoA) antibody produced in mouse
Brand: SIGMA

clone BAP-77, ascites fluid

Synonym(s):

Monoclonal Anti-Phosphatase, Alkaline, bacterial

About This Item

UNSPSC Code:

12352203

NACRES:

NA.46

MDL number:

MFCD02095875

Conjugate:

alkaline phosphatase conjugate

Clone:

BAP-77, monoclonal

Application:

ELISA (i), WB

Citations:

This product is discontinued. Contact Technical Service for assistance.

Properties

biological source

mouse

conjugate

alkaline phosphatase conjugate

antibody form

ascites fluid

antibody product type

primary antibodies

clone

BAP-77, monoclonal

mol wt

antigen ~50 kDa

species reactivity

bacteria

technique(s)

indirect ELISA: suitable, western blot: 1:20,000 using purified Escherichia coli BAP

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Description

General description

Bacterial alkaline phosphatase (BAP) is a periplasmic enzyme that is encoded by the phoA gene. The use of a BAP tag to the target gene yields a stable fusion product that does not affect the bioactivity and biodistribution of the target gene in recombinant expression vector systems. Hence, anti-BAP antibodies can be useful tools for the detection and analysis of target biomolecules fused to BAP tags.Monoclonal Anti-Bacterial Alkaline Phosphatase (BAP, PhoA) (mouse IgG1 isotype) is derived from the BAP-77 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a BAP tagged fusion protein. Bacterial alkaline phosphatase (BAP) also known as PhoA, EC 3.1.3.1 is an 50 kDa protein, derived from E. coli.

Immunogen

A bacterial alkaline phosphatase (BAP) tagged fusion protein.

Application

Monoclonal Anti-Bacterial Alkaline Phosphatase (BAP, PhoA) antibody is suitable for use in western blot (at 1:20,000 dilutions using purified Escherichia coli BAP) and indirect ELISA.

Biochem/physiol Actions

Bacterial alkaline phosphatase (BAP) enzyme regulates the catabolism of phosphate esters. Alkaline phosphatase appears to require export to the periplasm to show enzymatic activity. Fusions of the secreted alkaline phosphatase to an integral cytoplasmic membrane protein of E. coli shows different activities depending on where with the membrane protein the alkaline phosphatase is fused. Fusions to positions in or near the periplasmic (extracellular) domain lead to high alkaline phosphatase activity, whereas those to positions in the cytoplasmic domain give low activity.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	SAB4200860	A2951	A2080
Sigma-Aldrich B6804 Monoclonal Anti-Bacterial Alkaline Phosphatase (BAP, PhoA) antibody produced in mouse	Sigma-Aldrich SAB4200860 Anti-Bacterial Alkaline Phosphatase (BAP, PhoA) antibody, Mouse monoclonal	Sigma-Aldrich A2951 Monoclonal Anti-Alkaline Phosphatase, Human Placental antibody produced in mouse	Sigma-Aldrich A2080 Monoclonal Anti-Alkaline Phosphatase, Human Placental−Agarose antibody produced in mouse
description clone BAP-77, ascites fluid	description clone BAP-77, purified from hybridoma cell culture	description clone 8B6, ascites fluid	description clone 8B6, purified immunoglobulin, PBS suspension
conjugate alkaline phosphatase conjugate	conjugate -	conjugate unconjugated	conjugate agarose conjugate
clone BAP-77, monoclonal	clone BAP-77	clone 8B6, monoclonal	clone 8B6, monoclonal
biological source mouse	biological source -	biological source mouse	biological source mouse
antibody form ascites fluid	antibody form purified from hybridoma cell culture	antibody form ascites fluid	antibody form purified immunoglobulin
species reactivity bacteria	species reactivity E. coli (Strain K2)	species reactivity human	species reactivity human
shipped in dry ice	shipped in dry ice	shipped in dry ice	shipped in wet ice

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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