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Anti-Mouse IgG (Fab specific)–Biotin antibody produced in goat

Name: Anti-Mouse IgG (Fab specific)–Biotin antibody produced in goat
Brand: SIGMA

affinity isolated antibody, buffered aqueous solution

About This Item

UNSPSC Code:

12352203

NACRES:

NA.46

MDL number:

MFCD00162621

Conjugate:

biotin conjugate

Clone:

polyclonal

Application:

ELISA (d), IHC (p), WB

Citations:

This product is discontinued. Contact Technical Servicefor assistance.

Properties

biological source

goat

Quality Level

200

conjugate

biotin conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

direct ELISA: 1:200,000-1:300,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200, western blot: 1:400,000-1:800,000 using total cell extract of HeLa cells

storage temp.

−20°C

target post-translational modification

unmodified

Description

General description

IgG (immunoglobulin G) antibody subtype is a predominant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids. IgG is found in four subclasses IgG1, IgG2, IgG3 and IgG4. IgG has a monomeric structure.

The product binds to all mouse Igs

Application

Anti-Mouse IgG (Fab specific)-Biotin antibody produced in goat was used in immunohistochemistry of lateral and medial menisci of rabbits and human adipose-derived stem cells.

Biochem/physiol Actions

IgG (immunoglobulin G) antibody participates in complement fixation and opsonization.

IgG antibody provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of biotin to Anti-Mouse IgG (Fab specific) antibody allows for the binding of various labels such as avidin or streptavidin.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

Adsorbed to reduce background staining with bovine, horse or human samples.

Other Notes

Antibody adsorbed with bovine, equine and human serum proteins.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	B0529	B9904	B7401
Sigma-Aldrich B7151 Anti-Mouse IgG (Fab specific)–Biotin antibody produced in goat	Sigma-Aldrich B0529 Anti-Mouse IgG (Fab specific)–Biotin antibody produced in goat	Sigma-Aldrich B9904 Anti-Mouse IgG (Fc specific)–Biotin antibody produced in goat	Sigma-Aldrich B7401 Anti-Mouse IgG (Fc specific)–Biotin antibody produced in goat
biological source goat	biological source goat	biological source goat	biological source goat
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody
technique(s) direct ELISA: 1:200,000-1:300,000, western blot: 1:400,000-1:800,000 using total cell extract of HeLa cells, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200	technique(s) direct ELISA: 1:200,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:300, western blot: 1:300,000-1:600,000 using using Hela cells extract	technique(s) direct ELISA: 1:50,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:300, western blot: 1:200,000-1:400,000 using total cell extract of HeLa cells	technique(s) direct ELISA: 1:160,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400, western blot (chemiluminescent): 1:300,000 using total cell extract of HeLa cells (5-10 μg/well)
conjugate biotin conjugate	conjugate biotin conjugate	conjugate biotin conjugate	conjugate biotin conjugate
clone polyclonal	clone polyclonal	clone polyclonal	clone polyclonal
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C

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Storage Class

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number

0000453482

0000140665

0000312830

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The effects of BMP6 overexpression on adipose stem cell chondrogenesis: Interactions with dexamethasone and exogenous growth factors.

Brian O Diekman et al.

Journal of biomedical materials research. Part A, 93(3), 994-1003 (2009-09-02)

Adipose-derived stem cells (ASCs) are multipotent progenitors that can be chondrogenically induced by growth factors such as bone morphogenetic protein 6 (BMP-6). We hypothesized that nonviral transfection of a BMP-6 construct (pcDNA3-BMP6) would induce chondrogenic differentiation of ASCs encapsulated in

Functional properties of cell-seeded three-dimensionally woven poly(epsilon-caprolactone) scaffolds for cartilage tissue engineering.

Franklin T Moutos et al.

Tissue engineering. Part A, 16(4), 1291-1301 (2009-11-12)

Articular cartilage possesses complex mechanical properties that provide healthy joints the ability to bear repeated loads and maintain smooth articulating surfaces over an entire lifetime. In this study, we utilized a fiber-reinforced composite scaffold designed to mimic the anisotropic, nonlinear

Multifunctional hybrid three-dimensionally woven scaffolds for cartilage tissue engineering.