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B8563

Aniline Blue solution

2.5% in 2% acetic acid

Synonym(s):

Acid blue 93; Cl 42780

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About This Item

Empirical Formula (Hill Notation):
C32H25N3O9S3Na2
CAS Number:
28631-66-5
Molecular Weight:
737.73
MDL number:
MFCD00036130
UNSPSC Code:
41116121
NACRES:
NA.47
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form

liquid

Quality Level

200

concentration

2.5% in 2% acetic acid

technique(s)

microbe id | staining: suitable

color

dark blue

solubility

water: soluble

λmax

0.21-0.37 at 597-603 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

N(c2ccc(cc2)C(=C4C=CC(=Nc5ccccc5)C=C4)c3cc(c(cc3)N)C)c1ccccc1

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This Item
415049B8438318744
Aniline Blue solution 2.5% in 2% acetic acid

Sigma-Aldrich

B8563

Aniline Blue solution

Aniline Blue diammonium salt certified by the BSC

Sigma-Aldrich

415049

Aniline Blue diammonium salt

Alcian Blue solution 1% in 3% acetic acid, pH 2.5

Sigma-Aldrich

B8438

Alcian Blue solution

Bromophenol Blue solution 0.04 wt. % in H2O

Sigma-Aldrich

318744

Bromophenol Blue solution

technique(s)

microbe id | staining: suitable

technique(s)

-

technique(s)

microbe id | staining: suitable

technique(s)

titration: suitable

color

dark blue

color

brown to very dark brown, and Red to Very Dark Red and Red-Brown and Very Dark Red-Brown

color

dark blue to very dark blue

color

-

concentration

2.5% in 2% acetic acid

concentration

-

concentration

1% in 3% acetic acid, pH 2.5

concentration

0.04 wt. % in H2O

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

solubility

water: soluble

solubility

-

solubility

-

solubility

-

form

liquid

form

powder, crystals or granules

form

liquid

form

liquid

General description

Aniline Blue is the traditional name applied to anionic dyes produced by successive phenylation and sulfonation of basic fuchsine. Aniline blue consists of sulfonation products of variable mixtures of phenylated rosaniline and pararosaniline. It is a mixture of methyl blue and water blue. The dye is also known as cotton blue, and ink blue. Methyl blue of aniline blue belongs to the group of triaminotriphenylmethane dyes.

Application

Aniline blue (also known as cotton blue and ink blue) is a component of commonly used polychrome stains. It is used to stain collagen fibers in tissue sections using Masson′s trichrome protocol for staining multiple components. Collagen is stained blue by this method. The dye is suitable for selective staining of callose in plant specimens and staining histones for assessing nuclear maturity. Heidenhain′s AZAN stain and the Mallory′s polychrome stain both entail staining steps using aniline blue-orange G mixtures. Aniline blue is used in Gomori′s one-step trichrome stain and Mallory′s connective tissue stain for tissue including kidney and intestine.

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000532914
0000532355
0000526805
0000526805
0000526807

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Global Trade Item Number

SKUGTIN
B8563-500ML04061833443019
B8563-250ML04061833443002

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