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C0255

Collagenasi

Name: Collagenasi
Brand: SIGMA

purified by chromatography, ≥500 CDU/mg solid (CDU = collagen digestion units), lyophilized powder

Sinonimo/i:

Clostridiopeptidasi A

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Informazioni su questo articolo

Numero CAS:

9001-12-1

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-582-9

MDL number:

MFCD00130830

Numero CE:

3.4.24.3 (BRENDA, IUBMB)

Specific activity:

≥500 CDU/mg solid (CDU = collagen digestion units)

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Proprietà

form

lyophilized powder

Quality Segment

200

specific activity

≥500 CDU/mg solid (CDU = collagen digestion units)

mol wt

68-130 kDa

purified by

chromatography

solubility

TESCA buffer (50 mM TES, 0.36 mM Calcium chloride, pH 7.4): soluble 0.05-0.1 mg/mL at 37 °C

foreign activity

Clostripain ≤2.0 U/mg

storage temp.

2-8°C

Descrizione

General description

Collagenase from Clostridium histolyticum comprises of collagenases G and H. Collagenase has N-terminal activator domain and C-terminal peptidase domain. The collagen binding domains (CBD) and polycystic kidney disease-like (PKD-like) domain resides in the peptidase domain. The collagenase module undergoes structural changes upon calcium binding.

Application

Collagenase from Clostridium histolyticum may be used for the isolation of cells from many types of animal tissue. It may be used for the disaggregation of human tumor, mouse kidney, human adult/fetal brain, lung and many other tissues. It may also be used in liver and kidney perfusion studies, for the digestion of pancreas, isolation of nonparenchymal rat liver cells and for hepatocyte preparation.

Collagenase from Clostridium histolyticum has been used:

for the recovery of neutrophils from collagen gels[1]
for the dissociation of breast tumor biopsies into single cells
for the isolation of primary human endometrial stromal cells(HESC) from endometrial biopsies

Biochem/physiol Actions

La collagenasi è attivata da quattro grammi atomo di calcio per mole di enzima. È inibita da etilen-glicol-bis(beta-amminoetil etere) - acido N, N, N′,N′-tetraacetico, beta-mercaptoetanolo, glutatione, acido tioglicolico e 8-idrossichinolina.

Crude collagenases are mixtures of enzymes (mostly proteases) secreted by C. histolyticum. Effective release of cells from tissue requires the action of both collagenase enzymes and the neutral protease. Collagenase is activated by four gram atom calcium (Ca²⁺) per mole enzyme. The culture filtrate is thought to contain at least 7 different proteases ranging in molecular weight from 68-130 kDa.

Packaging

Product sold on the basis of collagen digestion units (CDU).

Preparation Note

Collagenase may be dissolved in TESCA buffer (50 mM TES, 0.36 mM Calcium chloride, pH 7.4 at 37 °C) at 0.05-0.1 mg/mL concentration.

Other Notes

Una unità di digestione del collagene (CDU) libera peptidi da collagene proveniente da tendine d′Achille bovino equivalente in colore ninidrina a 1,0 μmole di leucina in 5 ore a pH 7,4 a 37 °C in presenza di ioni calcio. Una unità di idrolisi FALGPA idrolizza 1,0 μmole di furilacriloil-Leu-Gly-Pro-Ala per minuto a 25 °C. Una unità di proteasi neutra idrolizza caseina e produce colore equivalente a 1,0 μmole di tirosina per 5 ore a pH 7,5 a 37 °C. Una unità di clostripaina idrolizza 1,0 μmole of BAEE per minuto a pH 7,6 a 25 °C in presenza di DTT.

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Questo articolo	C1889	C2139	C9407
Sigma-Aldrich C0255 Collagenasi	Sigma-Aldrich C1889 Collagenasi	Sigma-Aldrich C2139 Collagenasi	Sigma-Aldrich C9407 Collagenasi
description purified by chromatography, ≥500 CDU/mg solid (CDU = collagen digestion units), lyophilized powder	description 0.2 μm filtered, suitable for release of physiologically active rat hepatocytes, Type IV-S, 0.5-5.0 FALGPA units/mg solid, ≥125 CDU/mg solid	description suitable for release of rat epididymal adipocytes and hepatocytes (for methodology see Type II and Type IV), Type VIII, 0.5-5.0 FALGPA units/mg solid, ≥125 CDU/mg solid	description powder, Suitable for the digestion and isolation of physiologically active pancreatic islet cells, suitable for cell culture
specific activity ≥500 CDU/mg solid (CDU = collagen digestion units)	specific activity ≥125 CDU/mg solid, 0.5-5.0 FALGPA units/mg solid	specific activity ≥125 CDU/mg solid, 0.5-5.0 FALGPA units/mg solid	specific activity ≥800 units/mg solid
form lyophilized powder	form powder	form powder	form powder
solubility TESCA buffer (50 mM TES, 0.36 mM Calcium chloride, pH 7.4): soluble 0.05-0.1 mg/mL at 37 °C	solubility -	solubility -	solubility -
mol wt 68-130 kDa	mol wt 68-130 kDa	mol wt 68-125 kDa	mol wt 68-130 kDa
storage temp. 2-8°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
foreign activity Clostripain ≤2.0 U/mg	foreign activity -	foreign activity -	foreign activity -

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pictograms

GHS08,GHS07

signalword

Danger

hcodes

H315,H319,H334,H335

pcodes

P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Classe di stoccaggio

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c