Skip to Content
MilliporeSigma
Get 22% off for Pi Day through March 31.Save Now

Key Documents

View All Documentation

Skip To

C-22011

Endothelial Cell Growth Medium 2

Ready-to-use kit including Basal Medium and SupplementMix, 500 ml

Synonym(s):

Primary Cell Culture Media

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View
Pack SizeSKUAvailabilityPrice
500 mL
Check Cart for Availability
$222.00

About This Item

NACRES:
NA.71
UNSPSC Code:
12352207

$222.00

Check Cart for Availability
​
Request a Bulk Order
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

form

liquid

packaging

pkg of 500 ml

technique(s)

cell culture | mammalian: suitable

shipped in

wet ice

storage temp.

2-8°C

Looking for similar products? Visit Product Comparison Guide

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item
C-22111C-22211C-39211
Endothelial Cell Growth Medium 2 Ready-to-use kit including Basal Medium and SupplementMix, 500 ml

Sigma-Aldrich

C-22011

Endothelial Cell Growth Medium 2

Endothelial Cell Growth Medium 2 Kit including Basal Medium and SupplementPack, 500 ml

Sigma-Aldrich

C-22111

Endothelial Cell Growth Medium 2

Endothelial Cell Growth Medium 2 Basal Medium, 500 ml

Sigma-Aldrich

C-22211

Endothelial Cell Growth Medium 2

Endothelial Cell Growth Medium 2 SupplementPack containing all media supplements as individual vials, 1 Pack for 500 ml

Sigma-Aldrich

C-39211

Endothelial Cell Growth Medium 2

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

form

liquid

form

liquid

form

aqueous solution

form

liquid

packaging

pkg of 500 ml

packaging

pkg of 500 ml

packaging

pkg of 500 ml

packaging

pkg of 1 kit

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

dry ice

General description

Endothelial Cell Growth Medium 2 (Ready-to-use)

Application

Endothelial Cell Growth Medium is a low-serum (2% V/V) medium optimized for the cultivation of endothelial cells from large blood vessels. The media are optimized for primary human cells but can also be used for bovine, porcine, murine, caprine, rabbit, elephant, dog, and rat endothelial cells from large vessels as well as for various endothelial cell lines.Note: For best transfection results we recommend using PromoCell heparin-free Endothelial Cell Growth Medium MV2. Heparin, included all our other our Endothelial Cell Growth Media formulations, may reduce transfection efficiency.

Packaging

Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials

Preparation Note

Click here for more information.
Final supplement concentrations after addition to the basal medium:Epidermal Growth Factor (recombinant human): 5 ng / mlBasic Fibroblast Growth Factor (recombinant human): 10 ng / ml Insulin-like Growth Factor (Long R3 IGF): 20 ng / ml Vascular Endothelial Growth Factor 165 (recombinant human): 0.5 ng / mlAscorbic Acid: 1 µg / mlHeparin: 22.5 µg / ml Hydrocortisone: 0.2 µg / ml
Store the Basal Medium at 4 to 8°C in the dark and the SupplementMix or SupplementPack at -20°C immediately after arrival. Do not freeze the Basal Medium. If stored properly, the products are stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.

Analysis Note

All lots of PromoCell Endothelial Cell Media 2 are subjected to comprehensive quality control tests using primary human endothelial cells. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested endothelial cells. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Storage Class

10 - Combustible liquids

wgk

WGK 3

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Protocols

The Endothelial Cell Transwell Migration and Invasion Assay

Cell culture protocol: the endothelial cell transwell migration and invasion assay used to study angiogenesis and cancer cell metastasis. Explore over 350 PromoCell products.

Related Content

PromoCell® Primary Cells & Specialty Media

Explore expert protocols and culture media for human primary cell lines to replicate biological systems accurately.

Endothelial Cell Tube Formation Angiogenesis Assay

Cell culture protocol: the endothelial tube formation assay to study angiogenesis using HUVECs and other endothelial cell types. Explore over 350 PromoCell products.

Questions

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service