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C3921

Creatinase from Pseudomonas sp.

recombinant, expressed in E. coli, lyophilized powder, 10-15 units/mg protein

Synonym(s):

Creatine Amidinohydrolase

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About This Item

CAS Number:
37340-58-2
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
253-471-1
MDL number:
MFCD00130875
EC Number:
3.5.3.3 (BRENDA, IUBMB)
Specific activity:
10-15 units/mg protein
Recombinant:
expressed in E. coli
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recombinant

expressed in E. coli

form

lyophilized powder

specific activity

10-15 units/mg protein

mol wt

45 kDa

composition

Protein, ~70% biuret

storage temp.

2-8°C

Application

Creatine amidinohydrolase is a clinically important enzyme used in the determination of creatinine in blood and urine.

Biochem/physiol Actions

Creatinase is a homodimer with subunit molecular weights of approximately 45 kDa. It catalyzes the hydrolysis of creatine, in the presence of H2O, to form urea and sarcosine.

Other Notes

One unit will hydrolyze 1.0 μmole of creatine to urea and sarcosine per min at pH 7.5 at 37 °C.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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