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Anti-Caspase 9 antibody,Mouse monoclonal

Name: Anti-Caspase 9 antibody,Mouse monoclonal
Brand: SIGMA

clone CAS9, purified from hybridoma cell culture

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD01864176

Conjugate:

unconjugated

Clone:

CAS9, monoclonal

Application:

ARR, ELISA (i), WB

Citations:

This product is discontinued. Contact Technical Servicefor assistance.

Properties

biological source

mouse

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

CAS9, monoclonal

form

buffered aqueous solution

mol wt

antigen 37 kDa

species reactivity

human

concentration

~2 mg/mL

technique(s)

indirect ELISA: suitable, microarray: suitable, western blot: 0.5-1 μg/mL using a purified recombinant human caspase 9 preparation

isotype

IgG1

UniProt accession no.

P55211

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CASP9(842)

Description

General description

Monoclonal Anti-Caspase 9 (mouse IgG1 isotype) is derived from the CAS9 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a synthetic peptide corresponding to amino acids of human caspase 9 protein, conjugated to keyhole limpet hemocyanin (KLH). Caspases (cysteine-requiring aspartate protease) are a family of proteases that share similarities in amino acid sequences, structure and substrate specificity. Caspases are normally present in the cell as inactive procaspases. Caspase 9 (also known as Mch6 and ICE-LAP6) gene encodes a protein product of 37 kDa (reported also as 46 kDa), which undergoes a cleavage upon activation, leading to p18/p15/p9 subunits. It is a member of the CED-3 subfamily, bearing high similarity to caspase. High level expression of caspase 9 is found in the heart, testis and ovary.

Immunogen

synthetic peptide corresponding to amino acids 299-316 of human caspase 9 protein conjugated to KLH.

Application

Anti-Caspase 9 antibody,Mouse monoclonal has been used in:

western blot assay
immunocytochemistry
enzyme-linked immunosorbent assay (ELISA).

Biochem/physiol Actions

Caspase 9 is a downstream protease, activated in caspase 3- and granzyme B-mediated apoptosis. Procaspase 9 is activated when a complex is formed with the cofactor apoptotic protease activating factor-1 (Apaf-1) through the caspase recruitment domain (CARD). Caspase 9 plays a critical role in the survival of neuronal progenitors within the embryonic forebrain and cortex. Its deficiency leads to defective brain development and embryonic lethality. Overexpression of caspase 9, but not of a mutant in which the catalytic Cys was replaced with an Ala, induces apoptosis in MCF7 cells.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	SAB4300683	C7729	C8487
Sigma-Aldrich C4356 Anti-Caspase 9 antibody,Mouse monoclonal	Sigma-Aldrich SAB4300683 Anti-Caspase 9 antibody produced in rabbit	Sigma-Aldrich C7729 Anti-Caspase 9 antibody produced in rabbit	$Anti-Caspase 3, Active antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich C8487 Anti-Caspase 3, Active antibody produced in rabbit
species reactivity human	species reactivity human	species reactivity human, rat	species reactivity rat, bovine, human, pig, canine, mouse
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
biological source mouse	biological source rabbit	biological source rabbit	biological source rabbit
clone CAS9, monoclonal	clone polyclonal	clone polyclonal	clone polyclonal
antibody form purified from hybridoma cell culture	antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form IgG fraction of antiserum
Gene Information human ... CASP9(842)	Gene Information human ... CASP9(842)	Gene Information human ... CASP9(842) rat ... Casp9(58918)	Gene Information human ... CASP3(836) mouse ... Casp3(12367) rat ... Casp3(25402)

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Storage Class

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number

22170412

087M4802V

031K4890

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Role of caspases in the regulation of apoptotic pancreatic islet beta-cells death

Hui H, et al.

Journal of Cellular Physiology, 200(2), 177-200 (2004)

Apoptosis dependent and independent functions of caspases

Madame Curie Bioscience Database [Internet]. (2013)

Generation of Advanced Glycation End-Products (AGEs) by glycoxidation mediated by copper and ROS in a human serum albumin (HSA) model peptide: reaction mechanism and damage in motor neuron cells

Marques CMS, et al.

Mutation Research. Genetic Toxicology and Environmental Mutagenesis, 824(6), 42-51 (2017)

View All Related Papers

Global Trade Item Number

SKU	GTIN
C4356-.2ML	04061838104304

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