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C4405

Choline Oxidase from Arthrobacter globiformis

lyophilized powder, 8-20 units/mg solid

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Synonym(s):

Choline: oxygen 1-oxidoreductase

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100 units

Available to ship TODAYfromMILWAUKEE

$228.00
500 units

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$755.00
$641.75

About This Item

CAS Number:
9028-67-5
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-840-0
MDL number:
MFCD00130787
EC Number:
1.1.3.17 (BRENDA, IUBMB)
Specific activity:
8-20 units/mg solid

$228.00

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form

lyophilized powder

Quality Segment

specific activity

8-20 units/mg solid

mol wt

71 kDa by SDS-PAGE, 83 kDa by gel filtration

storage temp.

−20°C

General description

Research area: Cell signaling. Choline oxidaseis a flavoprotein and belongs to the glucose methanol choline(GMC)-oxidoreductase family.

Application

Choline oxidase from Sigma has been used in the enzymatic determination of choline in milk using a flow injection analysis (FIA) system with potentiometric detection.Choline oxidase from Arthrobacterglobiformis is suitable for the preparation of amperometric biosensors.
Choline Oxidase from Arthrobacter globiformis has been used:
  • for enzymatic determination of choline (Cho) and phosphocholine (P-Cho)
  • in sphingomyelin assay to determine the concentration of sphingomyelin by measuring free choline
  • in enzyme coating for the fully-assembled recording device for electrochemical measurements

Biochem/physiol Actions

Choline oxidase catalyzes the four-electron oxidation of choline to glycine-betaine, with betaine-aldehyde as intermediate and molecular oxygen as primary electron acceptor. The enzyme can also accept betaine-aldehyde as a substrate. This allows the investigation of the reaction mechanism for the conversion of choline to the aldehyde intermediate and of betaine-aldehyde to glycine-betaine. The enzyme is a flavoprotein with a molecular weight of approximately 83,000 Da according to gel filtration or approximately 71,000 Da according to SDS gel electrophoresis. The optimum pH is found to be around pH 7.5 and the isoelectric point (pI) around pH 4.5.

Other Notes

One unit will form 1 μmole of H2O2 with oxidation of 1 μmole of choline to betaine aldehyde per min at pH 8.0 at 37 °C. Note: During the conversion of choline to betaine by choline oxidase, 2 μmoles of H2O2 are produced for every μmole of choline.

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Choline Oxidase from Arthrobacter globiformis lyophilized powder, 8-20 units/mg solid

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Choline Oxidase from Arthrobacter sp.

Cholesterol Oxidase from microorganisms lyophilized powder, ≥50 units/mg protein, recombinant, expressed in E. coli

Sigma-Aldrich

C8868

Cholesterol Oxidase from microorganisms

description

lyophilized powder, 8-20 units/mg solid

description

lyophilized powder, ≥10 units/mg solid

description

recombinant, expressed in E. coli ≥12 units/mg protein

description

lyophilized powder, ≥50 units/mg protein, recombinant, expressed in E. coli

specific activity

8-20 units/mg solid

specific activity

≥10 units/mg solid

specific activity

≥12 units/mg protein

specific activity

≥50 units/mg protein

form

lyophilized powder

form

lyophilized powder

form

powder

form

lyophilized powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

mol wt

83 kDa by gel filtration, 71 kDa by SDS-PAGE

mol wt

~95 kDa

mol wt

-

mol wt

64 kDa

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Codes

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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    Certificates of Analysis (COA)

    Lot/Batch Number
    0000515244
    055K1574
    010M1981
    019K1509
    043K1158

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