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Anti-Calbindin-D-28K (KD-15) antibody produced in rabbit

Name: Anti-Calbindin-D-28K (KD-15) antibody produced in rabbit
Brand: SIGMA
Price: 599 USD
Availability: InStock

affinity isolated antibody, buffered aqueous solution

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Enhanced Validation

Synonym(s):

Anti-CALB, Anti-D-28K

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Size/SKU	Availability	Price
0.2 mL	Available to ship TODAYfromMILWAUKEE	$599.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.44

MDL number:

MFCD00164553

Conjugate:

unconjugated

Clone:

polyclonal

Application:

ARR, IHC (p), WB

Citations:

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Properties

biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 28 kDa

species reactivity

rat, chicken, human

enhanced validation

independent
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technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using sections of rat, human or chicken cerebellum sections, microarray: suitable, western blot: 1:1,000 using recombinant rat calbindin-D-28K

UniProt accession no.

P05937

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CALB1(793)
mouse ... Calb1(12307)
rat ... Calb1(83839)

Description

General description

Calbindin-D-28K (also termed vitamin D-dependent calcium-binding protein, or cholecalcin), is a highly conserved 28 kDa calcium binding protein, with broad tissue distribution. Itbelongs , to a family of low molecular weight calcium-binding proteins (CaBPs). Calbindin-D-28K is found predominantly in subpopulations of central and peripheral nervous system neurons, and in certain epithelial cells.

Immunogen

synthetic peptide corresponding to the C-terminal region of rat calbindin-D-28K (amino acids 185-199). This sequence is identical in the corresponding human, mouse and bovine calbindin-D-28K sequences and is highly conserved (single amino acid substitution) in chicken and frog calbindin-D-28K.

Application

Anti-Calbindin-D-28K (KD-15) antibody produced in rabbit has been used in:

immunocytochemistry
western blotting
microarray
immunohistochemistry

Biochem/physiol Actions

Calbindin-D-28K is an important neuroanatomical marker. Despite its broad tissue distribution, it exhibits a cell-type-specific expression pattern. It has been immunocytochemically localized in selected cells in hippocampus, where it is thought to buffer intracellular calcium, or transport intramembranous calcium.

Calbindin 1 (CALB) is a calcium-binding protein. The protein may play a role in development of Purkinje cells found in heterotopias and cerebellar dysgenesias. It also acts as a very important component of intracellular homeostasis in cerebellar neurons. The progressive decrease of calbindin content in the Purkinje cells undergoes degeneration and death during paraneoplastic changes in the cerebellum. Calbindin effects on Parkinson′s disease (PD) risk displays population specificity.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Analysis Note

Enzymatic predigestion of formalin-fixed, paraffin-embedded sections by proteolytic enzymes (e.g., 0.1% trypsin or protease, 10 min. at RT or 37 °C) improves immunohistochemical staining.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	C9848	C2724	C7479
Sigma-Aldrich C7354 Anti-Calbindin-D-28K (KD-15) antibody produced in rabbit	Sigma-Aldrich C9848 Monoclonal Anti-Calbindin-D-28K antibody produced in mouse	Sigma-Aldrich C2724 Anti-Calbindin-D-28K (EG-20) antibody produced in rabbit	Sigma-Aldrich C7479 Anti-Calretinin antibody produced in rabbit
description affinity isolated antibody, buffered aqueous solution	description clone CB-955, ascites fluid	description affinity isolated antibody, buffered aqueous solution	description affinity isolated antibody, buffered aqueous solution
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
antibody form affinity isolated antibody	antibody form ascites fluid	antibody form affinity isolated antibody	antibody form affinity isolated antibody
biological source rabbit	biological source mouse	biological source rabbit	biological source rabbit
form buffered aqueous solution	form -	form buffered aqueous solution	form buffered aqueous solution
shipped in dry ice	shipped in dry ice	shipped in dry ice	shipped in -

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Hazard Codes

H412

Precautionary Statements

P273

Hazard Classifications

Aquatic Chronic 3

Storage Class

10 - Combustible liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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