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Anti-Calnexin antibody, Mouse monoclonal

Name: Anti-Calnexin antibody, Mouse monoclonal
Brand: SIGMA
Price: 589 USD
Availability: InStock

Enhanced Validation

clone TO-5, purified from hybridoma cell culture

Synonym(s):

Anti-CANX, Anti-CNX

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Size/SKU	Availability	Price
200 μL	Available to ship TODAYfromMILWAUKEE	$589.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.44

MDL number:

MFCD01094399

Conjugate:

unconjugated

Clone:

TO-5, monoclonal

Application:

ELISA (i), FACS, ICC, IHC, WB

Citations:

$589.00

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Properties

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

TO-5, monoclonal

form

buffered aqueous solution

mol wt

antigen ~90 kDa

species reactivity

human

enhanced validation

independent
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concentration

~2 mg/mL

technique(s)

flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, indirect ELISA: suitable, western blot: 0.5-1.0 μg/mL using total cell extract of HeLa cells.

isotype

IgG1

UniProt accession no.

P27824

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CANX(821)

Description

General description

Anti-Calnexin antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma TO-5 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Calnexin contains a long (461 amino acids) N-terminal Ca²⁺-binding domain extending into the lumen of the endoplasmic reticulum (ER), a short (22 amino acids) trans-membrane segment and an acidic cytosolic domain (96 amino acids).

Calnexin (p88, IP90) is a calcium-binding, type I integral membrane protein, localized primarily in the endoplasmic reticulum (ER). Calnexin binds newly synthesized glycoproteins and misfolded proteins and is believed to play a critical role in quality control processes during protein synthesis and folding. Calnexin acts as a lectin like chaperone that binds oligosaccharide residues of newly synthesized N-linked glycoproteins. The lectin specificity of calnexin and its soluble homologue calreticulin has been identified as high mannose oligosaccharides terminating in monoglucosyl residues linked through α1-3. Calnexin has been shown to be associated with several cell surface proteins, including MHC class I heavy chain, T-cell receptor (TCR), and B cell membrane immunoglobulin during translocation through the ER. It also forms complexes with other resident ER proteins involved in Ca²⁺-dependent retention of proteins.

Application

Anti-Calnexin antibody, Mouse monoclonal has been used in:

enzyme-linked immunosorbent assay (ELISA)
immunoblotting[1]
flow cytometry
immunocytochemistry
immunohistochemistry
immunofluorescence analysis

Biochem/physiol Actions

Mouse monoclonal clone TO-5 anti-Calnexin antibody recognizes human Calnexin, ~90 kDa.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	SAB1402138	C4731	SAB4503258
Sigma-Aldrich C7617 Anti-Calnexin antibody, Mouse monoclonal	Sigma-Aldrich SAB1402138 Monoclonal Anti-CANX, (C-terminal) antibody produced in mouse	$Anti-Calnexin antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich C4731 Anti-Calnexin antibody produced in rabbit	Sigma-Aldrich SAB4503258 Anti-Calnexin, C-Terminal antibody produced in rabbit
description clone TO-5, purified from hybridoma cell culture	description clone 1D4, purified immunoglobulin, buffered aqueous solution	description IgG fraction of antiserum, buffered aqueous solution	description affinity isolated antibody
biological source mouse	biological source mouse	biological source rabbit	biological source rabbit
Gene Information human ... CANX(821)	Gene Information human ... CANX(821)	Gene Information human ... CANX(821) mouse ... Canx(12330) rat ... Canx(29144)	Gene Information human ... CANX(821)
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
species reactivity human	species reactivity human	species reactivity canine, rat, mouse, human	species reactivity mouse, rat, human
antibody form purified immunoglobulin	antibody form purified immunoglobulin	antibody form IgG fraction of antiserum	antibody form affinity isolated antibody
clone TO-5, monoclonal	clone 1D4, monoclonal	clone polyclonal	clone polyclonal

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c