C8274
Cytohelicase from Helix pomatia
lyophilized powder
Synonym(s):
Snail gut enzyme
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250 MG
CHF 497.00
CHF 497.00
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This Item | |||
|---|---|---|---|
| form lyophilized powder | form buffered aqueous solution | form buffered aqueous solution | form buffered aqueous solution |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| composition Protein, 50-80% Lowry | composition - | composition - | composition - |
Application
Cytohelicase from Helix pomatia has been used:
- in the enzyme solution for preparing the cell nuclei for immuno fluorescent in situ hybridization (immunoFISH)[1]
- in the enzyme mix to analyze meiotic cells at diakinesis and later stages in anthers[2]
- in the enzyme mix for the analysis of mitosis in anthers[3]
Cytohelicase from Helix pomatia has been used to study the isolation of yeast protoplasts using various preparations of the hepato-pancreatic juice of Helix pomatia.
The enzyme from Sigma has been used for cytohelicase assay. The enzyme assay was used while studying the effects of MNN10 gene-disruption on protein secretion in Kluyveromyces lactis and Saccharomyces cerevisiae cultures.[4]
Biochem/physiol Actions
Cytohelicase from Helix pomatia is used in the preparation of synaptonemal complex spreading techniques along with bovine serum albumin. It plays an important role in the digestion of cell wall.[5][6] Cytohelicase interacts with chromatin and improves the adhesion of synaptonemal complexes to the hydrophobic plastic film.[5]
Analysis Note
Contains glucanase/laminarinase activity
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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ImmunoFISH: simultaneous visualisation of proteins and DNA sequences gives insight into meiotic processes in nuclei of grasses.
Sepsi A, et al.
Frontiers in Plant Science, 9(7), 1193-1193 (2018)
Improved method of enzyme digestion for root tip cytology.
Lattier JD, et al.
Hortscience: a Publication of the American Society For Horticultural Science Hortscience, 52(7), 1029-1032 (2017)
The effect of bovine serum albumin and cytohelicase on surface-spread synaptonemal complexes of Rye (Secale cereale).
De Jong JH and van Eden J
Stain Technology, 63(4), 213-220 (1988)
Danguole Bartkeviciūte et al.
FEMS yeast research, 4(8), 833-840 (2004-09-29)
Screening for genes affecting super-secreting phenotype of the over-secreting mutant of Kluyveromyces lactis resulted in isolation of the gene named KlMNN10, sharing high homology with Saccharomyces cerevisiae MNN10. The disruption of the KlMNN10 in Kluyveromyces lactis, as well as of
Simon Amiard et al.
PloS one, 9(1), e86220-e86220 (2014-01-28)
In striking contrast to animals, plants are able to develop and reproduce in the presence of significant levels of genome damage. This is seen clearly in both the viability of plants carrying knockouts for key recombination and DNA repair genes
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