MilliporeSigma
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C8274

Sigma-Aldrich

Cytohelicase from Helix pomatia

lyophilized powder

Synonym(s):
Snail gut enzyme
CAS Number:
EC Number:
MDL number:

form

lyophilized powder

Quality Level

composition

Protein, 50-80% Lowry

storage temp.

2-8°C

Application

Cytohelicase from Helix pomatia has been used:
  • in the enzyme solution for preparing the cell nuclei for immuno fluorescent in situ hybridization (immunoFISH)[1]
  • in the enzyme mix to analyze meiotic cells at diakinesis and later stages in anthers[2]
  • in the enzyme mix for the analysis of mitosis in anthers[3]

Cytohelicase from Helix pomatia has been used to study the isolation of yeast protoplasts using various preparations of the hepato-pancreatic juice of Helix pomatia.
The enzyme from Sigma has been used for cytohelicase assay. The enzyme assay was used while studying the effects of MNN10 gene-disruption on protein secretion in Kluyveromyces lactis and Saccharomyces cerevisiae cultures.[4]

Packaging

250 mg in poly bottle

Biochem/physiol Actions

Cytohelicase from Helix pomatia is used in the preparation of synaptonemal complex spreading techniques along with bovine serum albumin. It plays an important role in the digestion of cell wall.[5][6] Cytohelicase interacts with chromatin and improves the adhesion of synaptonemal complexes to the hydrophobic plastic film.[5]

Analysis Note

Contains glucanase/laminarinase activity

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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