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Cholesterol Oxidase from Streptomyces sp.

Name: Cholesterol Oxidase from <I>Streptomyces</I> sp.
Brand: SIGMA
Price: 288 USD

lyophilized powder, ≥20 units/mg protein

Synonym(s):

Cholesterol: oxygen oxidoreductase

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Pack Size	SKU	Availability	Price
100 units		Check Cart for Availability	$288.00
250 units		Check Cart for Availability	$573.00

About This Item

CAS Number:

9028-76-6

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-842-1

MDL number:

MFCD00130783

EC Number:

1.1.3.6 (BRENDA, IUBMB)

Specific activity:

≥20 units/mg protein

Biological source:

Streptomyces sp.

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Properties

biological source

Streptomyces sp.

Quality Level

200

form

lyophilized powder

specific activity

≥20 units/mg protein

mol wt

~34 kDa

composition

protein, 55-65% biuret

solubility

50 mM potassium phosphate buffer, pH 7.0: soluble (Cold)

shipped in

dry ice

storage temp.

−20°C

Description

General description

Isoelectric point : 5.1 ± 0.1 and 5.4 ± 0.1
Michaelis constant : 4.3 x 10‾⁵M(Cholesterol)
Inhibitors : Ionic detergents, Hg⁺⁺, Ag⁺
Optimum pH : 6.5 − 7.0
Optimum temperature : 45 – 50°C
pH Stability : pH 5.0 – 10.0 (25°C, 20hr)
Thermal stability : Below 45°C (pH 7.0, 15min)

Cholesterol Oxidase produced from Streptomyces sp corresponds to a molecular weight of 62 kDa. It has a pH and temperature optimum of 7 and 37 °C, respectively.[1] Cholesterol Oxidase comprises a FAD-binding and a steroid-binding domain.

Application

Cholesterol oxidase from Streptomyces has been used in a study to assess the relationship between the micellar structure of model bile and the activity of esterase. Cholesterol oxidase from Streptomyces has also been used in a study to investigate the effects of sphingomyelin degradation on cell cholesterol oxidizability and steady-state distribution between the cell surface and the cell interior.

Cholesterol oxidase is used to determine serum cholesterol.[2][3] The enzyme also finds application in the microanalysis of steroids in food samples and in distinguishing 3-ketosteroids from 3β-hydroxysteroids.[4] Transgenic plants expressing cholesterol oxidase are being investigated in the fight against the cotton boll weevil.[5] CHOD has also been used as a molecular probe to elucidate cellular membrane structures.[6][7]

Biochem/physiol Actions

Cholesterol oxidase (CHOD) is a monomeric flavoprotein containing FAD that catalyzes the first step in cholesterol catabolism. This bifunctional enzyme oxidizes cholesterol to cholest-5-en-3-one in an FAD-requiring step, which is then isomerized to cholest-4-en-3-one with the release of H₂O₂.[8]

Cholesterol Oxidase from microorganisms use cholesterol for carbon and energy. It is useful in diagnostic assays involving cholesterol, especially in lipid disorders. Cholesterol oxidase has an insecticidal property and aids protection against boll weevil larvae. The Streptomyces sp cholesterol oxidase has long shelf-life with good performance.

Physical form

lyophilized powder containing bovine serum albumin and amino acids as stabilizers

Preparation Note

CHOD is soluble in cold 50 mM potassium phosphate buffer, pH 7.0. Prepare solutions immediately before use.

Other Notes

One unit will convert 1.0 μmole of cholesterol to 4-cholesten-3-one per min at pH 7.5 at 25 °C. Note: 4-cholesten-3-one may undergo isomerization.

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This Item	228250	C5421	C1235
Sigma-Aldrich C8649 Cholesterol Oxidase from Streptomyces sp.	Sigma-Aldrich 228250 Cholesterol Oxidase, Streptomyces sp.	Sigma-Aldrich C5421 Cholesterol Oxidase from microorganisms	Sigma-Aldrich C1235 Cholesterol Oxidase microbial
specific activity ≥20 units/mg protein	specific activity ≥3 units/mg solid, ≥40 U/mg	specific activity ≥30 units/mg protein (biuret)	specific activity ≥10 units/mg protein
biological source Streptomyces sp.	biological source -	biological source -	biological source -
form lyophilized powder	form lyophilized solid	form aqueous solution	form lyophilized powder
storage temp. −20°C	storage temp. −20°C	storage temp. −70°C	storage temp. −20°C
shipped in dry ice	shipped in ambient	shipped in dry ice	shipped in -
solubility 50 mM potassium phosphate buffer, pH 7.0: soluble (Cold)	solubility water: 20 mg/mL	solubility 50 mM potassium phosphate buffer, pH 7.0: soluble (Cold)	solubility 50 mM potassium phosphate buffer, pH 7.0: soluble