Key Documents

View All Documentation

HT 1080

Name: HT 1080
Brand: SIGMA

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 85111505, human fibrosarcoma, Epithelial

Synonym(s):

HT1080 Cells

Select a Size

Change View

Size/SKU	Availability	Price

About This Item

UNSPSC Code:

41106514

Biological source:

human fibrosarcoma

Relevant disease(s):

cancer

Growth mode:

Adherent

Karyotype:

2n = 46, pseudodiploid

Morphology:

Epithelial

Manufacturer/tradename:

ECACC

Pricing and availability is not currently available.

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

biological source

human fibrosarcoma

manufacturer/tradename

ECACC

packaging

tube of 5 μg 85111505-DNA-5UG, pkg of vial of cells 85111505-1VL

growth mode

Adherent

karyotype

2n = 46, pseudodiploid

morphology

Epithelial

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Description

Application

HT 1080 has been used to demonstrate that type I collagen induces matrix metallopeptidase 2 (MMP-2) activation. It has also been used to study the roles of Ras-related C3 botulinum toxin substrate 1 and 2 (Rac1 and Rac2) in regulating morphology, F-actin organization, adhesion, migration and chemotaxis of HT1080 cells.

Biochem/physiol Actions

Established from a fibrosarcoma arising adjacent to the acetabulum of a 35 year old caucasian male. The cells can give rise to tumours in immunosupressed NIH Swiss mice. Susceptible to infection by RNA tumour viruses including RD114 feline endogenous virus and FeLV.

Human fibrosarcoma

STR-PCR Data: Amelogenin: X,Y,
CSF1PO: 12
D13S317: 12,14
D16S539: 9,12
D5S818: 11,13
D7S820: 9,10
THO1: 6
TPOX: 8
vWA: 14,19

Preparation Note

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:4 to 1:8 i.e. seeding at 1-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item	CB_89050201	CB_90112810	CB_94011416
HT 1080	Hs 633T	HSDM1C1	RT 1
description NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 85111505, human fibrosarcoma, Epithelial	description NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.	description NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.	description NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
relevant disease(s) cancer	relevant disease(s) cancer	relevant disease(s) cancer	relevant disease(s) cancer
storage temp. −196°C	storage temp. −196°C	storage temp. −196°C	storage temp. −196°C
packaging tube of 5 μg 85111505-DNA-5UG, pkg of vial of cells 85111505-1VL	packaging tube of 5 μg 89050201-DNA-5UG, pkg of vial of cells 89050201-1VL	packaging tube of 5 μg 90112810-DNA-5UG, pkg of vial of cells 90112810-1VL	packaging tube of 5 μg 94011416-DNA-5UG, pkg of vial of cells 94011416-1VL
karyotype 2n = 46, pseudodiploid	karyotype Not specified	karyotype 2n = 40	karyotype Modal no. 40
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
biological source human fibrosarcoma	biological source human fibrosarcoma	biological source mouse fibrosarcoma	biological source rat unknown/unspecified

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.