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3,5-Dinitrosalicylic acid

Name: 3,5-Dinitrosalicylic acid
Brand: SIGMA
Price: 51 CAD
Availability: InStock

used in colorimetric determination of reducing sugars

Synonym(s):

3,5-Dinitro-2-hydroxybenzoic acid, DNS

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Pack Size	Availability	Price
10 g	Estimated to ship onMay 11, 2026fromMilliporeSigma Canada Ltd.	$51.00
25 g	Estimated to ship onMay 11, 2026fromMilliporeSigma Canada Ltd.	$92.50
100 g	Estimated to ship onMay 11, 2026fromMilliporeSigma Canada Ltd.	$196.00
500 g	Please contact Customer Service for Availability	$871.00

About This Item

Linear Formula:

(O₂N)₂C₆H₂-2-(OH)CO₂H

CAS Number:

609-99-4

Molecular Weight:

228.12

UNSPSC Code:

12171500

NACRES:

NA.47

PubChem Substance ID:

24893243

EC Number:

210-204-3

Beilstein/REAXYS Number:

2220661

MDL number:

MFCD00007104

Assay:

≥98% (HPLC and titration)

Form:

powder

$51.00

Estimated to ship onMay 11, 2026Details

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Properties

Quality Level

200

assay

≥98% (HPLC and titration)

form

powder

mp

168-172 °C (lit.)

solubility

H₂O: 50 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

OC(=O)c1cc(cc(c1O)[N+]([O-])=O)[N+]([O-])=O

InChI

1S/C7H4N2O7/c10-6-4(7(11)12)1-3(8(13)14)2-5(6)9(15)16/h1-2,10H,(H,11,12)

InChI key

LWFUFLREGJMOIZ-UHFFFAOYSA-N

Description

Application

3,5-Dinitrosalicylic acid (DNS) has been used to stop the reaction in in vitro α-amylase inhibition study.[1] It has also been used for the quantitation of enzymatically released reducing sugars.

Biochem/physiol Actions

3,5-Dinitrosalicylic acid (DNS) is used in colorimetric determination of reducing sugars[2] and to analyze glycosidase (glycoside hydrolase) activity by quantitation of enzymatically released reducing sugar. The dinitrosalicylic acid method has been compared to the Nelson-Somogi colorimetric method.

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Show Differences

1 of 1

This Item	128848	D124001	136883
Sigma-Aldrich D0550 3,5-Dinitrosalicylic acid	Sigma-Aldrich 128848 3,5-Dinitrosalicylic acid	Sigma-Aldrich D124001 3,5-Diiodosalicylic acid	Sigma-Aldrich 136883 4-Methyl-3,5-dinitrobenzoic acid
assay ≥98% (HPLC and titration)	assay 98%	assay 99%	assay 98%
Quality Level 200	Quality Level 200	Quality Level 100	Quality Level 100
solubility H₂O: 50 mg/mL	solubility -	solubility -	solubility methanol: soluble 250 mg/10 mL, clear, faintly yellow to yellow
storage temp. room temp	storage temp. -	storage temp. -	storage temp. -
mp 168-172 °C (lit.)	mp 168-172 °C (lit.)	mp 220-230 °C (dec.) (lit.)	mp 155-158 °C (lit.)
form powder	form powder	form powder	form solid

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Explore all of our products under 3,5-Dinitrosalicylic acid

pictograms

GHS05,GHS07

signalword

Danger

hcodes

H302,H318

pcodes

P264 - P270 - P280 - P301 + P312 - P305 + P351 + P338 - P501

Hazard Classifications

Acute Tox. 4 Oral - Eye Dam. 1

Storage Class

4.1A - Other explosive hazardous materials

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Protocols

Enzymatic Assay of α-Amylase (EC 3.2.1.1)

Follow our procedure for the determination of alpha-Amylase activity. This enzymatic assay of a-Amylase guides you through the entire process and necessary calculations.

Studies on the ecology of actinomycetes in an agricultural soil amended with organic residues: II. Assessment of enzymatic activities of Actinomycetales isolates.

Mokni-Tlili S, et al.

World Journal of Microbiology & Biotechnology, 27, 2251-2259 (2011)

Comparison of two methods for assaying reducing sugars in the determination of carbohydrase activities.

Gusakov, A.V., et al.

International Journal of Antimicrobial Agents, e283658-e283658 (2011)

Use of dinitrosalicylic acid reagent for determination of reducing sugar.

Miller, G.L.

Analytical Chemistry, 31, 426-428 (1959)

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Global Trade Item Number

SKU	GTIN
D0550-500G	04061835347377
D0550-25G	04061835347360
D0550-100G	04061835347346
D0550-10G	04061835347353

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