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D7679

3,3′-Diaminobenzidine (DAB) Liquid Substrate Dropper System

liquid

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1 set
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$410.00

About This Item

CAS Number:
NACRES:
NA.75
PubChem Substance ID:
UNSPSC Code:
12352204
EC Number:
231-018-9
MDL number:

$410.00


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form

liquid

Quality Level

technique(s)

immunoblotting: suitable

solubility

water: soluble

shipped in

wet ice

storage temp.

2-8°C

SMILES string

Cl.Cl.Cl.Cl.Nc1ccc(cc1N)-c2ccc(N)c(N)c2

InChI

1S/C12H14N4.4ClH/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8;;;;/h1-6H,13-16H2;4*1H

InChI key

KJDSORYAHBAGPP-UHFFFAOYSA-N

General description

DAB (3,3′-Diaminobenzidine Tetrahydrochloride) Liquid Substrate Dropper System has been developed for use in immunohistology and immunoblotting procedures as a precipitating substrate for the detection of peroxidase activity. DAB is the immunohistology substrate of choice because it produces an intense brown stain that is easily observed. The end product is resistant to alcohol, therefore, a variety of counterstains and mounting media can be used with the DAB Liquid Substrate Dropper System. DAB Liquid Substrate Dropper System provides all of the chromogen and buffer/peroxide solutions needed to produce a fast and convenient DAB substrate solution.

Application

3,3′-Diaminobenzidine (DAB) Liquid Substrate Dropper System has been used as a developer in immunohistogical examination.

Preparation Note

Each DAB liquid substrate dropper system will provide ten individual application dropper bottles of 10 mL of a fast and convenient DAB substrate solution.

Disclaimer

Stable at least one year at 2-8 °C.

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This Item
D7304D6815D5637
technique(s)

immunoblotting: suitable

technique(s)

-

technique(s)

-

technique(s)

titration: 96-104% (with NaOH)

solubility

water: soluble

solubility

-

solubility

-

solubility

H2O: 50 mg/mL

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

liquid

form

liquid

form

liquid

form

powder, crystals or chunks

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C


Kit Components Only

Product No.
Description

  • 10× DAB Liquid Chromogen 10 mL

  • premeasured, ready-to-use buffer/peroxide solution for individual application 10 x 9

wgk

WGK 3

Storage Class

3 - Flammable liquids

pictograms

Flame

signalword

Danger

hcodes

Hazard Classifications

Flam. Liq. 2

flash_point_f

51.8 °F - closed cup

flash_point_c

11 °C - closed cup



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Related Content

Instructions


S M Hsu et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 30(10), 1079-1082 (1982-10-01)
Three metallic ions, NiCl2, CoCl2, and CuSO4, were found to modify the color of the normally brown diaminobenzidine (DAB) reaction. The colors ranged from purplish blue (NiCl2), dark blue/bluish black (CoCl2), to greyish blue (CuSO4). We have found that the
The duality of beta-catenin function: a requirement in lens morphogenesis and signaling suppression of lens fate in periocular ectoderm
Smith AN, et al.
Developmental Biology, 285(2), 477-489 (2005)
Carola W N Damen et al.
Analytical biochemistry, 393(1), 73-79 (2009-06-16)
For the bioanalysis of therapeutic monoclonal antibodies in biological matrices, immunoassays--especially enzyme-linked immunosorbent assays (ELISAs)--are the most widely used techniques. Although ELISAs are very sensitive, the obtained sensitivity is not always sufficient. In this study, we have investigated the possibilities



Global Trade Item Number

SKUGTIN
D7679-1SET04061833588741

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