E5900
Anti-c-erbB-4 antibody, Mouse monoclonal
clone HER4-36, purified from hybridoma cell culture
Synonym(s):
Anti-HER-4
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About This Item
Conjugate:
unconjugated
Clone:
HER4-36, monoclonal
Application:
ARR, IP
Citations:
3
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
HER4-36, monoclonal
form
buffered aqueous solution
mol wt
antigen 180-185 kDa
species reactivity
human
concentration
~2 mg/mL
technique(s)
immunoprecipitation (IP): 4-8 μg/test using RIPA lysate of cultured CB4 cells (CHO cell line transfected with erbB-4), microarray: suitable
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... ERBB4(2066)
General description
Monoclonal Anti-c-erbB-4 (erb-b2 receptor tyrosine kinase 4) (HER-4) (mouse IgG1 isotype) is derived from the HER4-36 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a purified extracellular domain of human erbB-4 from transfected cells. The human ErbB-4 oncogene (also known as HER4) is a 180–185 kDa receptor that binds to a family of isoforms, collectively known as neuregulins. This gene belongs to epidermal growth factor receptor family and is expressed mainly in several breast carcinoma cell lines, heart, brain, cerebellum, pituitary and in normal skeletal muscle.
Immunogen
extracellular domain of human erbB-4 from transfected cells.
Application
Anti-c-erbB-4 antibody, Mouse monoclonal has been used in immunoprecipitation. It has also been used in studies on receptor turnover, phosphorylation, ligand binding and induction of differentiation of cultured breast cancer cells.
Monoclonal anti-c-erbB-4 antibody can be used in immunoprecipitation (4-8 μg/test) using RIPA lysate of cultured CB4 cells (CHO cell line transfected with erbB-4). It can also be used in microarray.
Biochem/physiol Actions
The epidermal growth factor receptor (ErbB/HER) family of transmembrane receptor tyrosine kinases significantly participates in defining cell lineage during mesenchyme-epithelial inductive processes and in the neuro-muscular and glia-Schwann cells interactions. It plays a crucial role as a cell surface receptor for neuregulins. It can also bind with erbB -2 to forms heterodimers and can modulate ligand affinity. The catalytic domain is important for the intrinsic tyrosine kinase activity and shows greater degree of homology. Certain ligands and receptors of the family contribute to a relatively virulent phenotype of some human tumors; most notable are carcinomas of secretory epithelia. ErbB-4 also binds to related group of molecules, termed neuregulin 2 (NRG2)(which also binds to erbB-3) and NRG3 (which binds exclusively to erbB-4). Two other ligands, betacellulin and the heparin-binding EGF-like growth factor, bind to both erbB-1 and erbB-4. ErbB-4 forms heterodimers with erbB-2, resulting in the modulation of its ligand affinity.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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This Item | |||
|---|---|---|---|
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate unconjugated |
| species reactivity human | species reactivity human | species reactivity human, rat, mouse | species reactivity rat, mouse, human |
| antibody form purified immunoglobulin | antibody form affinity isolated antibody | antibody form purified immunoglobulin | antibody form purified immunoglobulin |
| biological source mouse | biological source rabbit | biological source mouse | biological source mouse |
| clone HER4-36, monoclonal | clone polyclonal | clone MEKK4-338, monoclonal | clone RNP1, monoclonal |
| Gene Information human ... ERBB4(2066) | Gene Information human ... ERBB2(2064) | Gene Information human ... MAP3K4(4216) | Gene Information human ... RAF1(5894) |
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Storage Class
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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Related Content
Instructions
Use of cigarette-smoking history to estimate the likelihood of mutations in epidermal growth factor receptor gene exons 19 and 21 in lung adenocarcinomas
Pham D, et al.
Journal of Clinical Oncology, 24(11), 1700-1704 (2006)
An immunological approach reveals biological differences between the two NDF/heregulin receptors, ErbB-3 and ErbB-4
Chen X, et al.
The Journal of Biological Chemistry, 271(13), 7620-7629 (1996)
R Goldman et al.
Biochemistry, 29(50), 11024-11028 (1990-12-18)
The erbB-1 and erbB-2 protooncogenes encode homologous membrane receptors that respectively bind epidermal growth factor (EGF) and a still incompletely characterized ligand. Binding of EGF to its receptor is known to increase tyrosine phosphorylation of the erbB-2/neu receptor in tumor
Global Trade Item Number
| SKU | GTIN |
|---|---|
| E5900-.2ML | 04061838105141 |