F1392
Riboflavin 5′-monophosphate sodium salt hydrate
suitable for electrophoresis, suitable for acrylamide photopolymerization, ≥70%, powder
Synonyme(s) :
FMN-Na, Flavin mononucleotide, Riboflavin 5′-phosphate sodium salt
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| À vous/Référence | Disponibilité | Prix |
|---|---|---|
25 mg | Vérifier la disponibilité des articles du panier | 89.70 CHF |
A propos de cet article
89.70 CHF
Quality Segment
assay
≥70%
form
powder
optical activity
[α]20/D +37 to +42°, c = 1.5 in 5 M HCl(lit.)
technique(s)
electrophoresis: suitable
solubility
H2O: soluble 50 mg/mL, clear, orange
ε (extinction coefficient)
12.5 at 445 nm at 1 mM
suitability
suitable for acrylamide photopolymerization
foreign activity
Protease, none detected
storage temp.
−20°C
SMILES string
O.[Na+].Cc1cc2N=C3C(=O)NC(=O)N=C3N(C[C@H](O)[C@H](O)[C@H](O)COP(O)([O-])=O)c2cc1C
InChI
1S/C17H21N4O9P.Na.H2O/c1-7-3-9-10(4-8(7)2)21(15-13(18-9)16(25)20-17(26)19-15)5-11(22)14(24)12(23)6-30-31(27,28)29;;/h3-4,11-12,14,22-24H,5-6H2,1-2H3,(H,20,25,26)(H2,27,28,29);;1H2/q;+1;/p-1/t11-,12+,14-;;/m0../s1
InChI key
BHRVCJBIICJWTH-APQIITSESA-M
Application
- to study its mechanism of inhibition of mutagenicity of a benzo[a]pyrene 7,8-diol 9,10-epoxide, a carcinogenic metabolite of benzo[a]pyrene
- along with flavin adenine dinucleotide for reactivation of the enzyme that catalyzes the oxidation of pyridoxamine to pyridoxal in a study
- to study the reduction of polymeric azo and nitro dyes by intestinal bacteria
- to study the effect of FMN on ferrisiderophore reductase activity in the cytoplasmic fraction of Agrobacterium tumefaciens.
Biochem/physiol Actions
FMN is suitable as a photopolymerization reagent in polyacrylamide gel electrophoresis (PAGE) by forming free radicals in aqueous solution in the presence of light. FMN photodecomposes to leucoflavin. No free radicals are formed in the absence of oxygen, but traces of oxygen allow for leucoflavin to reoxidize with free-radical generation. The catalysts, tetramethylethylenediamine (TEMED) or 3-dimethylaminopropionitrile (DMAPN), are commonly added to speed up the free radical formation. Free radicals will cause acrylamide and bis-acrylamide to polymerize to form a gel matrix which can be used for sieving macromolecules. FMN is commonly used in the stacking gel for non-denaturing PAGE because native proteins can be sensitive to persulfate ions from ammonium persulfate. Another advantage of FMN over ammonium persulfate is that it will not start polymerizing until the gel is illuminated.
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Cet article | |||
|---|---|---|---|
| description suitable for electrophoresis, suitable for acrylamide photopolymerization, ≥70%, powder | description tested according to Ph. Eur. | description European Pharmacopoeia (EP) Reference Standard | description synthetic, ≥70% (HPLC) |
| Quality Level 100 | Quality Level 200 | Quality Level - | Quality Level 200 |
| form powder | form powder | form solid | form powder |
| assay ≥70% | assay - | assay - | assay ≥70% (HPLC) |
| solubility H2O: soluble 50 mg/mL, clear, orange | solubility water: soluble 100 g/L at 20 °C | solubility - | solubility water: 50 mg/mL |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C |
| optical activity [α]20/D +37 to +42°, c = 1.5 in 5 M HCl(lit.) | optical activity - | optical activity - | optical activity - |
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Classe de stockage
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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