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F7512

Anti-Rabbit IgG (whole molecule)−FITC antibody produced in sheep

Name: Anti-Rabbit IgG (whole molecule)−FITC antibody produced in sheep
Brand: SIGMA

affinity isolated antibody, buffered aqueous solution

Synonym(e):

Sheep Anti-Rabbit IgG (whole molecule)−Fluorescein isothiocyanate

zur Ansicht der Organisations- und Vertragspreise.

Über diesen Artikel

UNSPSC Code:

12352203

NACRES:

NA.46

MDL number:

MFCD00162786

Conjugate:

FITC conjugate

Clone:

polyclonal

Application:

IF (d), IHC (p)

Citations:

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Eigenschaften

biological source

sheep

conjugate

FITC conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

storage condition

protect from light

technique(s)

direct immunofluorescence: 1:160, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:160

storage temp.

−20°C

target post-translational modification

unmodified

Beschreibung

General description

Rabbit IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in rabbit serum. IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection.
Fluorescein isothiocyanate (FITC) is a fluorescein derivative (fluorochrome) used to tag antibodies, including secondary antibodies, for use in fluorescence-based assays and procedures. FITC excites at 495 nm and emits at 521 nm.

Sheep polyclonal anti-Rabbit IgG (whole molecule)−FITC antibody binds normal rabbit serum and rabbit IgG. It does not cross react with human IgG.

Application

Anti-Rabbit IgG (whole molecule)-FITC antibody produced in sheep has been used in:

immunocytochemistry
immunofluorescence
immunohistochemistry

Sheep polyclonal anti-Rabbit IgG (whole molecule)−FITC antibody may be used to detect and quantitate the level of IgG in rabbit serum and biological fluids by fluorescent techniques.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Other Notes

Antibody adsorbed with human IgG.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Dieser Artikel	F5137	F0382	F4512
Sigma-Aldrich F7512 Anti-Rabbit IgG (whole molecule)−FITC antibody produced in sheep	$Anti-Sheep IgG (whole molecule)−FITC antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich F5137 Anti-Sheep IgG (whole molecule)−FITC antibody produced in rabbit	Sigma-Aldrich F0382 Anti-Kaninchen-IgG (Gesamtmolekül)-FITC in Ziege hergestellte Antikörper	$Anti-Human IgG (whole molecule)−FITC antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich F4512 Anti-Human IgG (whole molecule)−FITC antibody produced in rabbit
technique(s) direct immunofluorescence: 1:160, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:160	technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100, indirect immunofluorescence: 1:100	technique(s) indirect immunofluorescence: 1:80	technique(s) immunofluorescence: 1:64-1:128 using Hep2 cells
conjugate FITC conjugate	conjugate FITC conjugate	conjugate FITC conjugate	conjugate FITC conjugate
antibody form affinity isolated antibody	antibody form IgG fraction of antiserum	antibody form affinity isolated antibody	antibody form IgG fraction of antiserum
biological source sheep	biological source rabbit	biological source goat	biological source rabbit
form buffered aqueous solution	form buffered aqueous solution	form buffered aqueous solution	form buffered aqueous solution
clone polyclonal	clone polyclonal	clone polyclonal	clone polyclonal

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