F8146
Anti-Factor VII antibody, Mouse monoclonal
clone HVII-1, purified from hybridoma cell culture
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 0.2 mL | Please contact Customer Service for Availability | $839.00 |
About This Item
Conjugate:
unconjugated
Clone:
HVII-1, monoclonal
Application:
ELISA (i), WB
Citations:
6
$839.00
Please contact Customer Service for Availability
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
HVII-1, monoclonal
form
buffered aqueous solution
species reactivity
human
technique(s)
indirect ELISA: suitable, western blot: 1:4,000
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... F7(2155)
General description
Anti-Factor VII antibody, mouse monoclonal (mouse) IgG1 isotype) is derived from the HVII-1 hybridoma produced by the fusion of mouse Sp2/0-Ag14 myeloma cells and splenocytes from immunized BAB/c mice. Human coagulation factor VII contains 10 gamma-carboxyglutamic acid residues (Gla), located at the N-terminal region of the molecule. The activated factor VII (factor VIIa) also consists of a light chain with the Gla-domain and an epidermal growth factor domain, which is linked by a disulfide bond to a heavy chain, containing the serine protease catalytic domain.
Immunogen
Factor VII from pooled normal human plasma.
Application
Anti-Factor VII antibody, Mouse monoclonal has been used in:
- the preparation of factor VII - depleted plasma and for purification
- sandwich-type immunoassays
- the evaluation of patients with hereditary factor VII deficiency by an enzyme linked immunosorbent assay (ELISA) or radio immunosorbent assay (RIA)
- the assay of factor VII level in patients with liver disease (a sensitive parameter of liver dysfunction)
- epidemiological studies of the importance of factor VII level as a risk factor for coronary heart disease, cerebrovascular disease and peripheral vascular diseases
- the determination of the activity state of factor VII in in vivo samples when used with a clotting assay
- flow cytometry
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Flow cytometry/Cell sorting (1 paper)
Flow cytometry/Cell sorting (1 paper)
Monoclonal Anti-Factor VII antibody is suitable for flow cytometry analysis to study the behavior of TF-fVIIa secreted by ovarian cancer cells was associated with microparticles (MPs). It is also suitable for western blot at a dilution of 1:4,000 and indirect ELISA.
Biochem/physiol Actions
Factor VII is a single-chain vitamin K-dependent glycoprotein in human coagulation. It has a molecular weight of ∼50,000Da, present in plasma in trace quantities. It is predominantly synthesized in liver and depends on the posttranslational γ−carboxylation of specific glutamic residues located near the NH2-terminus of the molecule. It is a serine protease zymogen and for activation it requires proteolytic cleavage in the disulfide-linked, two-chain form, Factor VIIa, by Factor Xa, Factor IXa, thrombin and Factor XIIa or β XIIa.
The antibody recognizes an epitope on the light chain of Factor VII in the presence or absence of divalent cations. Clone no. HVII-1 (also cited as clone no. MC1476/E.A.8.1) may be used for purification of Factor VII and for the preparation of Factor VII depleted human plasma.
Physical form
Solution in 10 mM HEPES buffer, pH 7.4, with 140 mM sodium chloride and 0.05% sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
1 of 1
This Item | |||
|---|---|---|---|
| antibody form purified immunoglobulin | antibody form purified immunoglobulin | antibody form purified from hybridoma cell culture | antibody form purified immunoglobulin |
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate unconjugated |
| species reactivity human | species reactivity human | species reactivity human | species reactivity bovine, human |
| biological source mouse | biological source mouse | biological source mouse | biological source mouse |
| Gene Information human ... F7(2155) | Gene Information human ... F9(2158) | Gene Information human ... F9(2158) | Gene Information human ... FGF2(2247) |
| clone HVII-1, monoclonal | clone HIX-1, monoclonal | clone HIX-5, monoclonal | clone FB-8, monoclonal |
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Storage Class
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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Related Content
Instructions
Regulated readthrough: A new method for the alternative tagging and targeting of recombinant proteins
Bouquin T, et al.
Journal of Biotechnology, 125(4), 516-528 (2006)
Structural biology of factor VIIa/tissue factor initiated coagulation
Vadivel K and Bajaj SP
Frontiers in Bioscience, 17, 2476-2476 (2012)
G J Broze et al.
The Journal of biological chemistry, 255(4), 1242-1247 (1980-02-25)
Blood coagulation Factor VII was purified 100,000-fold from fresh frozen human plasma to apparent homogeneity with a yield of 30% based on coagulation assay. The molecular weight estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis was 48,000. Factor VII is composed
Global Trade Item Number
| SKU | GTIN |
|---|---|
| F8146-.2ML | 04061838105370 |