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F8771

Anti-Mouse IgG (Fab specific)−FITC antibody produced in goat

Name: Anti-Mouse IgG (Fab specific)−FITC antibody produced in goat
Brand: SIGMA

affinity isolated antibody, buffered aqueous solution

Sinônimo(s):

Goat Anti-Mouse IgG (Fab specific)−Fluorescein isothiocyanate

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Sobre este item

UNSPSC Code:

12352203

NACRES:

NA.46

MDL number:

MFCD00162622

Conjugate:

FITC conjugate

Clone:

polyclonal

Application:

IF, IHC (p)

Citations:

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Propriedades

biological source

goat

conjugate

FITC conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

storage condition

protect from light

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200, indirect immunofluorescence: 1:64

storage temp.

−20°C

target post-translational modification

unmodified

Descrição

General description

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections.

Immunogen

Purified mouse IgG

Application

Anti-Mouse IgG (Fab specific)-FITC antibody may be used for immunofluorescence of human peripheral blood lymphocytes at a minimum dilution of 1:64. It may be used for immunohistochemistry of formalin-fixed, paraffin-embedded human tonsil slides at a working antibody dilution of 1:200. For immunofluorescence, a working dilution of 1:200 was used in various studies. To study DNA synthesis in mouse two-cell stage embryos by BrdU antibody, mouse IgG (Fab specific)-FITC antibody dilution of 1:50 was used.

Biochem/physiol Actions

Anti-Mouse IgG (Fab specific)-FITC antibody is specific for Fab fragment of all mouse IgG subclasses. It shows no reactivity with mouse IgG, Fc fragment, or human IgG. Goat anti-Mouse IgG is conjugated to FITC and then purified by gel filtration to remove free FITC. The antibody is adsorbed with human IgG and rat serum proteins to ensure minimal cross reactivity.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

Adsorbed to reduce background with human or rat samples

Other Notes

Antibody adsorbed with human IgG and rat serum proteins.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Este Item	F4018	F9006	F5262
Sigma-Aldrich F8771 Anti-Mouse IgG (Fab specific)−FITC antibody produced in goat	Sigma-Aldrich F4018 Anti-Mouse IgG (Fab specific)−FITC antibody produced in goat	$Anti-Mouse IgG (whole molecule)–FITC antibody produced in goat IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich F9006 Anti-Mouse IgG (whole molecule)–FITC antibody produced in goat	Sigma-Aldrich F5262 Anti-Mouse IgG (Fab specific)−FITC antibody produced in goat
conjugate FITC conjugate	conjugate FITC conjugate	conjugate FITC conjugate	conjugate FITC conjugate
biological source goat	biological source goat	biological source goat	biological source goat
technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200, indirect immunofluorescence: 1:64	technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200, indirect immunofluorescence: 1:64	technique(s) direct immunofluorescence: 1:64	technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:150, indirect immunofluorescence: 1:128
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form IgG fraction of antiserum	antibody form affinity isolated antibody
storage temp. −20°C	storage temp. −20°C	storage temp. 2-8°C	storage temp. −20°C
form buffered aqueous solution	form buffered aqueous solution	form buffered aqueous solution	form buffered aqueous solution

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Classe de armazenamento

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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