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F9012

Anti-Goat IgG (whole molecule)–FITC antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD00162348
Conjugate:
FITC conjugate
Clone:
polyclonal
Application:
IF, IHC (p)
Citations:
8

biological source

rabbit

conjugate

FITC conjugate

antibody form

IgG fraction of antiserum

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:160, indirect immunofluorescence: 1:200

storage temp.

−20°C

target post-translational modification

unmodified

General description

The immunoglobulin G (IgG) is a major class of immunoglobulin and is highly conserved. IgG constitutes to 10−20% of the plasma proteins. This class of immunoglobulins are glycoproteins containing 82−96% protein and the remaining, carbohydrate. It exists in four subclasses namely, IgG1, IgG2, IgG3, and IgG4. IgG is composed of four polypeptide chains containing two heavy and two light chains, linked by disulfide bonds.

Application

Anti-Goat IgG (whole molecule)-FITC antibody produced in rabbit has been used for immunocytochemistry of human oocytes and preimplantation embryos.
It has also been used in enzyme linked immunosorbent assay (ELISA) and confocal microscopy.[1]

Biochem/physiol Actions

Deficiency of a particular IgG subclass, may increase the susceptibility towards certain pathogens.
IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of FITC to Anti- Goat IgG (whole molecule) antibody allows for the visualization of protein by fluorescent microscopy.
The antiserum is determined to be immunospecific for goat IgG.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Anti-Goat IgG (whole molecule)–FITC antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution

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F9012

Anti-Goat IgG (whole molecule)–FITC antibody produced in rabbit

Anti-Goat IgG (whole molecule)–FITC antibody produced in rabbit affinity isolated antibody, buffered aqueous solution

Sigma-Aldrich

F7367

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Anti-Rat IgG (whole molecule)–FITC antibody produced in rabbit affinity isolated antibody, buffered aqueous solution

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Anti-Rat IgG (whole molecule)–FITC antibody produced in rabbit

Anti-Goat IgG (whole molecule)–TRITC antibody produced in rabbit affinity isolated antibody, buffered aqueous solution

Sigma-Aldrich

T7028

Anti-Goat IgG (whole molecule)–TRITC antibody produced in rabbit

conjugate

FITC conjugate

conjugate

FITC conjugate

conjugate

FITC conjugate

conjugate

TRITC conjugate

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:160, indirect immunofluorescence: 1:200

technique(s)

direct immunofluorescence: 1:400, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:320, indirect immunofluorescence: 1:320

technique(s)

direct immunofluorescence: 1:100, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100

antibody form

IgG fraction of antiserum

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

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Storage Class

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

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Certificates of Analysis (COA)

Lot/Batch Number
029M4816V
21181208
088M4791V
21180252

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Related Content

Data Sheet - F9012

Instructions

Peter N Pushparaj et al.
Journal of immunology (Baltimore, Md. : 1950), 183(2), 1413-1418 (2009-07-01)
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Indian Journal of Animal Sciences (India) , 7 null
G Cauffman et al.
Molecular human reproduction, 11(3), 173-181 (2005-02-08)
The transcription factor OCT-4 is regarded as a critical factor in controlling mammalian early embryonic development because of its role in toti-/pluripotency. In human preimplantation embryos, OCT-4 studies are limited to RNA analysis of abnormally developing embryos. This study thoroughly
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Global Trade Item Number

SKUGTIN
F9012-1ML04061837517280

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