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Anti-FLIPγ/δ, C-Terminal antibody produced in rabbit

Name: Anti-FLIPγ/δ, C-Terminal antibody produced in rabbit
Brand: SIGMA

1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-CASH, Anti-CLARP, Anti-FLAME-1, Anti-I-FLICE

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD02096351

Conjugate:

unconjugated

Clone:

polyclonal

Application:

ICC, IF, WB

Citations:

This product is discontinued. Contact Technical Service for assistance.

Properties

biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 25 kDa (FLIPδ/FLIP_S), antigen 35 kDa (FLIPγ)

species reactivity

human, mouse

concentration

1 mg/mL

technique(s)

immunocytochemistry: 2 μg/mL, immunofluorescence: 10 μg/mL, western blot: 1:1,000

UniProt accession no.

O15519

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CFLAR(8837)
mouse ... Cflar(12633)

Description

General description

In human Viral FLICE-inhibitory proteins (v-FLIPs) is identified as c-FLIP. It is composed of two death effector domains which have structural resemblance with the N-terminal half of caspase-8 and a caspase-like domain. It exist as multiple splice variants: FLIP α, β, γ and δ. Along with splice variants, it has two endogenous forms of the protein − c-FLIP_long and c-FLIP_short.

Immunogen

FLIP antibody was raised against a 19 amino acid peptide near the carboxy terminus of human FLIP.
The immunogen is located within amino acids 180 - 230 of FLIP.

Application

Anti-FLIPγ/δ, C-Terminal antibody is suitable for microarray and western blot at a dilution of 1:1,000 using HeLa, Jurkat, THP-1, A431, K562, and NIH-3T3 cell lysates.

Biochem/physiol Actions

C-FLIP plays an important role in apoptosis signaling pathways. It acts as proapoptotic molecule or as an anti-apoptotic molecule. It has been reported that c-FLIP can interact with both FADD and caspase-8. It prevents caspase-8 recruitment and processing through DED-DED (Death Effector Domain) interaction followed by CD95-induced apoptosis.

Physical form

Solution in 0.01 M phosphate buffered saline containing 0.02% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	SAB4200477	AV00022	A2231
Sigma-Aldrich F9925 Anti-FLIPγ/δ, C-Terminal antibody produced in rabbit	Sigma-Aldrich SAB4200477 Anti-ALIX (C-terminal) antibody produced in rabbit	$Anti-CFLAR antibody produced in rabbit IgG fraction of antiserum$ Sigma-Aldrich AV00022 Anti-CFLAR antibody produced in rabbit	Sigma-Aldrich A2231 Anti-Axin1 (C-terminal region) antibody produced in rabbit
description 1 mg/mL, affinity isolated antibody, buffered aqueous solution	description ~1.0 mg/mL, affinity isolated antibody	description IgG fraction of antiserum	description ~2.5 mg/mL, affinity isolated antibody, buffered aqueous solution
biological source rabbit	biological source rabbit	biological source rabbit	biological source rabbit
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
Quality Level 200	Quality Level 200	Quality Level 100	Quality Level -
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form IgG fraction of antiserum	antibody form affinity isolated antibody
Gene Information human ... CFLAR(8837) mouse ... Cflar(12633)	Gene Information human ... PDCD6IP(10015) mouse ... Pdcd6ip(18571)	Gene Information human ... CFLAR(8837)	Gene Information human ... AXIN1(8312) mouse ... Axin1(12005) rat ... Axin1(79257)
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C

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10 - Combustible liquids

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