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F9925

Anti-FLIPγ/δ, C-Terminal antibody produced in rabbit

1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-CASH, Anti-CLARP, Anti-FLAME-1, Anti-I-FLICE

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD02096351
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ICC, IF, WB
Citations:
10

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 25 kDa (FLIPδ/FLIPS), antigen 35 kDa (FLIPγ)

species reactivity

human, mouse

concentration

1 mg/mL

technique(s)

immunocytochemistry: 2 μg/mL, immunofluorescence: 10 μg/mL, western blot: 1:1,000

UniProt accession no.

O15519

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CFLAR(8837)
mouse ... Cflar(12633)

General description

In human Viral FLICE-inhibitory proteins (v-FLIPs) is identified as c-FLIP. It is composed of two death effector domains which have structural resemblance with the N-terminal half of caspase-8 and a caspase-like domain. It exist as multiple splice variants: FLIP α, β, γ and δ. Along with splice variants, it has two endogenous forms of the protein − c-FLIPlong and c-FLIPshort.

Immunogen

FLIP antibody was raised against a 19 amino acid peptide near the carboxy terminus of human FLIP.
The immunogen is located within amino acids 180 - 230 of FLIP.

Application

Anti-FLIPγ/δ, C-Terminal antibody is suitable for microarray and western blot at a dilution of 1:1,000 using HeLa, Jurkat, THP-1, A431, K562, and NIH-3T3 cell lysates.

Biochem/physiol Actions

C-FLIP plays an important role in apoptosis signaling pathways. It acts as proapoptotic molecule or as an anti-apoptotic molecule. It has been reported that c-FLIP can interact with both FADD and caspase-8. It prevents caspase-8 recruitment and processing through DED-DED (Death Effector Domain) interaction followed by CD95-induced apoptosis.

Physical form

Solution in 0.01 M phosphate buffered saline containing 0.02% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item
AV00022HPA019044WH0008837M1
Anti-FLIPγ/δ, C-Terminal antibody produced in rabbit 1 mg/mL, affinity isolated antibody, buffered aqueous solution

Sigma-Aldrich

F9925

Anti-FLIPγ/δ, C-Terminal antibody produced in rabbit

Anti-CFLAR antibody produced in rabbit IgG fraction of antiserum

Sigma-Aldrich

AV00022

Anti-CFLAR antibody produced in rabbit

Anti-CFLAR antibody produced in rabbit affinity isolated antibody, buffered aqueous glycerol solution

Sigma-Aldrich

HPA019044

Anti-CFLAR antibody produced in rabbit

Monoclonal Anti-CFLAR antibody produced in mouse clone 2F12, purified immunoglobulin, buffered aqueous solution

Sigma-Aldrich

WH0008837M1

Monoclonal Anti-CFLAR antibody produced in mouse

antibody form

affinity isolated antibody

antibody form

IgG fraction of antiserum

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

Quality Level

200

Quality Level

100

Quality Level

100

Quality Level

100

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

mouse

UniProt accession no.

O15519

UniProt accession no.

O15519

UniProt accession no.

O15519

UniProt accession no.

O15519

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous glycerol solution

form

buffered aqueous solution

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Storage Class

10 - Combustible liquids

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Certificates of Analysis (COA)

Lot/Batch Number
SLBZ4696
SLBD4941
SLBB1750
097K1467
090K1005

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Related Content

M Thome et al.
Nature, 386(6624), 517-521 (1997-04-03)
Viruses have evolved many distinct strategies to avoid the host's apoptotic response. Here we describe a new family of viral inhibitors (v-FLIPs) which interfere with apoptosis signalled through death receptors and which are present in several gamma-herpesviruses (including Kaposi's-sarcoma-associated human
Apoptosis. Placing death under control.
D Wallach
Nature, 388(6638), 123-123 (1997-07-10)
H B Shu et al.
Immunity, 6(6), 751-763 (1997-06-01)
Caspases are cysteine proteases that play a central role in apoptosis. Caspase-8 may be the first enzyme of the proteolytic cascade activated by the Fas ligand and tumor necrosis factor (TNF). Caspase-8 is recruited to Fas and TNF receptor-1 (TNF-R1)
View All Related Papers

Global Trade Item Number

SKUGTIN
F9925-.1MG04061833420706

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