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Anti-Gα _i3 antibody produced in rabbit

Name: Anti-Gα <SUB>i3</SUB> antibody produced in rabbit
Brand: SIGMA
Price: 1880 USD
Availability: InStock

whole antiserum, buffered aqueous solution

Synonym(s):

Anti-87U6, Anti-ARCND1, Anti-HG1A

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Size/SKU	Availability	Price
1 vial	Check Cart for Availability	$1,880.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD00239679

Conjugate:

unconjugated

Clone:

polyclonal

Application:

IP, WB

Citations:

$1,880.00

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Properties

biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

whole antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~40 kDa

species reactivity

mouse, human, rat, hamster

technique(s)

immunoprecipitation (IP): suitable, western blot: 1:1,000-1:2,000 using solubilized mouse and rat brain membrane lysates, and mouse 3T3 cell lysates

UniProt accession no.

P08754

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... GNAI3(2773)
mouse ... Gnai3(14679)
rat ... Gnai3(25643)

Description

General description

G-proteins are membrane associated heterotrimeric proteins that are comprised of α-, β-, and λ-subunits. These proteins play a key role in signal transduction pathways that regulate cell′s response to several hormones, neuromodulators and ligands.

Immunogen

synthetic peptide corresponding to the C-terminal region of Gα_i3.

Application

Anti-Gα i3 antibody produced in rabbit is suitable for immunoprecipitation at a working antibody amount of 4μL using solubilized mouse brain membrane lysates and immunoblotting at a working dilution of 1:2000 using solubilized mouse and rat brain membrane lysates, and mouse 3T3 cell lysates.

Biochem/physiol Actions

Gi α 3 is a G-protein subunit that is involved in many signal transduction pathways including the mediation of EGF-induced PLC-γ activation and Ca⁺² mobilization in hepatocytes. The α-subunit contains a guanine-binding domain that is in its inactive state when it is occupied by GDP. Upon activation, GDP is replaced with GTP, causing the dissociation of the α-subunit from the βλ-subunit complex. This enables the Gα-GTP complex to bind to and regulate specific signaling pathways. GTP is then hydrolyzed, allowing for re-association of the α-subunit with the βλ-subunit complex.

Recognizes Gα_i3 and Gα₀ G-proteins.

Physical form

Solution of whole antiserum diluted in PBS, pH 7.4.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Show Differences

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This Item	SAB2109109	SAB4501225	MAB3073
Sigma-Aldrich G4040 Anti-Gα _i3 antibody produced in rabbit	Sigma-Aldrich SAB2109109 Anti-GNAI3 antibody produced in rabbit	Sigma-Aldrich SAB4501225 Anti-GZ-α antibody produced in rabbit	Sigma-Aldrich MAB3073 Anti-G Protein Goα Antibody, clone 2A
description whole antiserum, buffered aqueous solution	description affinity isolated antibody	description affinity isolated antibody	description clone 2A, Chemicon^®, from mouse
Quality Level 200	Quality Level -	Quality Level 100	Quality Level 100
antibody form whole antiserum	antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form purified immunoglobulin
biological source rabbit	biological source rabbit	biological source rabbit	biological source mouse
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
Gene Information human ... GNAI3(2773) mouse ... Gnai3(14679) rat ... Gnai3(25643)	Gene Information human ... GNAI3(57396)	Gene Information human ... GNAZ(2781)	Gene Information human ... GNAO1(2775)
mol wt antigen ~40 kDa	mol wt 39 kDa	mol wt antigen 40 kDa	mol wt -

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Storage Class

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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