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L2137

Lipopolysaccharides from Salmonella enterica serotype minnesota

Name: Lipopolysaccharides from <I>Salmonella enterica</I> serotype minnesota
Brand: SIGMA

purified by gel-filtration chromatography

Synonyme(s) :

LPS

pour consulter les tarifs organisationnels et contractuels.

A propos de cet article

UNSPSC Code:

12352201

NACRES:

NA.25

MDL number:

MFCD00131520

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Propriétés

biological source

Salmonella enterica (Serotype minnesota)

Quality Segment

200

form

lyophilized powder

purified by

gel-filtration chromatography

impurities

<1% Protein

color

white

solubility

water: 4.90-5.10 mg/mL, cloudy to hazy, colorless

shipped in

ambient

storage temp.

2-8°C

Description

General description

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

This product is extracted from Salmonella Minnesota and purified by gel filtration. The source strain is ATCC 9700. Mechanisms of complement activation by LPS of both rough and smooth strains of Salmonella Minnesota were studied by immunoassay of the activation products.

Application

TRPV4 is not the molecular sensor for bacterial lipopolysaccharides-induced calcium signaling: This study explores the signaling pathways activated by lipopolysaccharides from Salmonella enterica serotype Minnesota, demonstrating that TRPV4 is not involved, advancing understanding of immune responses to bacterial endotoxins (Wang et al., 2023).

Biochem/physiol Actions

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

Preparation Note

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 ^oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Other Notes

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Comparer avec des articles similaires

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1 of 1

Cet article	L6261	L2012	L7770
Sigma-Aldrich L2137 Lipopolysaccharides from Salmonella enterica serotype minnesota	Sigma-Aldrich L6261 Lipopolysaccharides from Salmonella enterica serotype minnesota	Sigma-Aldrich L2012 Lipopolysaccharides from Salmonella enterica serotype enteritidis	Sigma-Aldrich L7770 Lipopolysaccharides from Salmonella enterica serotype enteritidis
biological source Salmonella enterica (Serotype minnesota)	biological source Salmonella enterica (serotype minnesota)	biological source Salmonella enterica (Serotype enteritidis)	biological source Salmonella enterica (Serotype enteritidis)
solubility water: 4.90-5.10 mg/mL, cloudy to hazy, colorless	solubility water: soluble	solubility water: 4.90-5.10 mg/mL, cloudy to hazy, colorless to faintly yellow	solubility water: 4.90-5.10 mg/mL, faintly hazy to hazy, colorless
form lyophilized powder	form lyophilized powder	form lyophilized powder	form lyophilized powder
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
impurities <1% Protein	impurities <3% Protein (Lowry)	impurities <1% Protein	impurities <1% Protein (Lowry)
color white	color -	color white to faint yellow	color white to yellow cast

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Classe de stockage

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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