This enzyme is a crude acetone preparation that may contain many other enzymes, proteins, and cellular debris. The Km and Vmax of this product have not been determined.
Fortfahren mit
L2157
Laccase aus Rhus vernicifera
crude acetone powder, ≥50 units/mg solid
Synonym(e):
Benzoldiol:Sauerstoff-Oxidoreduktase
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Über diesen Artikel
CAS-Nummer:
UNSPSC Code:
12352204
EC Number:
420-150-4
NACRES:
NA.54
MDL number:
Specific activity:
≥50 units/mg solid
form
crude acetone powder
specific activity
≥50 units/mg solid
greener alternative product characteristics
Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
greener alternative category
storage temp.
−20°C
SMILES string
[n]1(c(c(cc1C)C=O)C)CC
InChI
1S/C9H13NO/c1-4-10-7(2)5-9(6-11)8(10)3/h5-6H,4H2,1-3H3
InChI key
NWDZDFOKSUDVJV-UHFFFAOYSA-N
General description
Wir verpflichten uns, Ihnen umweltfreundlichere Alternativprodukte anzubieten, die einen oder mehrere der „12 Prinzipien der Grünen Chemie“ erfüllen. Dieses Produkt wurde im Hinblick auf Energieeffizienz und Abfallvermeidung für die Verwendung in der Biobrennstoffzellen- und Celluloseethanol-Forschung verbessert. Weitere Informationen finden Sie in dem Artikel in Biofiles sowie unter Enzyme für die alternative Energieforschung.
Other Notes
Eine Einheit erzeugt ein ΔA530 von 0,001 pro min bei pH 6,5 und 30 °C in einem 3-mL-Reaktionsvolumen unter Verwendung von Syringaldazin als Substrat.
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Lagerklasse
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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What are the Km and Vmax of this laccase L2157?
1 answer-
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What is the temperature optimum of Product L2157, Laccase from Rhus vernicifera?
1 answer-
Our own assay is performed at 30°C. One publication (Casella et al., J. Inorg. Biochem., 100, 2127-2139 (2006)) gives 25°C as another value for the same enzyme from the same species.
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What is the pH optimum of Product L2157, Laccase from Rhus vernicifera?
1 answer-
Our own assay is performed at pH 6.5. One publication (Casella et al., J. Inorg. Biochem., 100, 2127-2139 (2006)) gives pH 6.5 as another value for the laccase from this species, with a range for enzyme activity of 4.3 to 9.
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What is the molecular weight (MW) of Product L2157, Laccase from Rhus vernicifera?
1 answer-
Spira-Solomon and Solomon, J. Am. Chem. Soc., 109, 6421-6432 (1987) reports estimated molecular weights of 52 kDa (laccase 1), 55 kDa (laccase 2), and 70 kDa (laccase 3), as determined by gel filtration.
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My solution of Product L2157, Laccase from Rhus vernicifera, isn't clear. Is this normal?
1 answer-
Yes. L2157 is a crude acetone powder, and can contain other proteins, enzymes, and cellular debris. A suspension may be prepared in 100 mM potassium phosphate buffer, pH 6.5, by homogenization with a hand-held homogenizer for 10 minutes. This preparation may then be centrifuged; the laccase activity will be present in the supernatant.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How stable are solutions of Product L2157, Laccase from Rhus vernicifera?
1 answer-
Solutions should be prepared fresh, directly before use, as they are not stable as stock solutions.
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