M5437
Anti-MSK-1 antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
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About This Item
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ARR, ICC, WB
Citations:
9
Pricing and availability is not currently available.
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 90 kDa
species reactivity
rat
technique(s)
immunocytochemistry: 1:1,000 using methanol/acetone-fixed rat fibroblast Rat-1 cell line, microarray: suitable, western blot: 1:2,000 using using whole cell lysate of rat fibroblast Rat-1 cell line
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... RPS6KA5(9252)
rat ... Rps6ka5(314384)
General description
The mitogen- and stress-activated kinases, MSK-1 and MSK-2 are activated by ERK/MAPK and p38 MAPK in response to growth and cell stress stimuli. MSK-1 and MSK-2 have 75% homology and interact with ERK1/2 and p38 through MAPK-binding domains near the C-terminus. There is a predominant expression of MSK proteins in heart, brain, skeletal muscles, lung, liver, kidney, pancreas and placenta. Highly conserved phosphorylation sites of MSK-1, Ser260 and Thr581, are followed by proline residues indicating that MSK-1 is activated by more than one MAPK. Endogenous MSK-1 is activated in cells by growth factors, phorbol ester stimulation, exposure to UV irradiation, and oxidative or chemical stress and is localized to nucleus of unstimulated or stimulated cells. MSK-1 is directly involved in the regulation of gene expression since it actively phosphorylates, stabilizes and activates many transcription factors. The major transcription factors that are regulated by MSK-1 include CREB, NF-κB and STAT3. MSKs also evidently phosphorylate Histone H3 at Ser10 and Ser28 and HMG at Ser6. Thus a primary role of MSK proteins is also promotion of gene relaxation and activation by regulating the binding of transcription repressors. Anti-MSK-1 specifically recognizes rat MSK-1 (90 kDa).
Immunogen
synthetic peptide corresponding to the C-terminus of human MSK1 (amino acids 783-800). This sequence is not found in the human and mouse MSK2 isoforms.
Application
Anti-MSK-1 may be used at a minimum working dilution of 1:2000 (immunoblotting in whole cell extracts) or 1:1000 (immunocytochemistry) in fibroblast Rat1 cell line. The antobody has also been used for detection by immunoblotting in mouse fibroblast 10T1/2 cells.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
1 of 1
This Item | |||
|---|---|---|---|
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate unconjugated |
| biological source rabbit | biological source mouse | biological source rabbit | biological source mouse |
| antibody form IgG fraction of antiserum | antibody form purified immunoglobulin | antibody form IgG fraction of antiserum | antibody form purified immunoglobulin |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
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Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Related Content
Instructions
Y Zhang et al.
The Journal of biological chemistry, 276(45), 42534-42542 (2001-09-13)
Phosphorylation of Tyr(705) and Ser(727) of signal transducer and activator of transcription 3 (STAT3) are known to be required for maximal activation by diverse stimuli. Tyr(705) phosphorylation is generally accepted to be mediated by the Janus kinase family. But the
Linda Vermeulen et al.
Trends in biochemical sciences, 34(6), 311-318 (2009-05-26)
Among the mitogen-activated protein kinase (MAPK) targets, MSKs (mitogen- and stress-activated protein kinases) comprise a particularly interesting protein family. Because MSKs can be activated by both extracellular-signal-regulated kinase and p38 MAPKs, they are activated by many physiological and pathological stimuli.
S Thomson et al.
The EMBO journal, 18(17), 4779-4793 (1999-09-02)
The nucleosomal response refers to the rapid phosphorylation of histone H3 on serine 10 and HMG-14 on serine 6 that occurs concomitantly with immediate-early (IE) gene induction in response to a wide variety of stimuli. Using antibodies against the phosphorylated
Global Trade Item Number
| SKU | GTIN |
|---|---|
| M5437-.2ML | 04061837913884 |