There has not been any conduct suitability testing for Nile Red. Please see the following for an article that may offer information; J. Histochem. Cytochem. 35:619-621 reference.
To perform the staining, a stock solution of Nile red was prepared by dissolving 10 mg of Nile red in 10 ml of acetone, which was then stored in the refrigerator protected from light. The sections were fixed in 1% glutaraldehyde in PBS for 5 minutes and washed in PBS three times for 2 minutes each. The staining solution was then prepared by diluting 100 ul of the stock solution in 1 ml of PBS. The sections were covered with this solution for 20 minutes at room temperature, then briefly washed in PBS and mounted in 75% glycerol in PBS. Other sections were incubated with solutions containing 50, 10, and 1 ug and 100 ng of Nile red per ml. The sections were examined with a filter set for fluorescein fluorescence (450-500 nm band pass excitation filter, a 510 nm center wavelength chromatic beam splitter, and a 528 nm long-pass barrier filter) and were photographed.
N3013
Nile Red
Powder
Synonym(s):
9-(diethylamino)-5H-benzo[a]phenoxazin-5-one, 9-(diethylamino)benzo[a]phenoxazin-5-one, Nile Blue A Oxazone
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| Size/SKU | Availability | Price |
|---|---|---|
100 mg | Available to ship TODAYfromBangalore Non-Bonded Warehouse | ₹15,371.50 |
1 g | Available to ship TODAYfromBangalore Non-Bonded Warehouse | ₹73,447.63 |
About This Item
Product Name
Nile Red, Technical grade
grade
technical grade
Quality Segment
form
powder
composition
Carbon: 70.0-77.3%
Nitrogen: 7.5-8.8%
technique(s)
titration: suitable
mp
203-205 °C (lit.)
solubility
methanol: 1 mg/mL
λmax
553 nm
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
CCN(CC)c1ccc2N=C3C(Oc2c1)=CC(=O)c4ccccc34
InChI
1S/C20H18N2O2/c1-3-22(4-2)13-9-10-16-18(11-13)24-19-12-17(23)14-7-5-6-8-15(14)20(19)21-16/h5-12H,3-4H2,1-2H3
InChI key
VOFUROIFQGPCGE-UHFFFAOYSA-N
General description
Application
- identification of microplastics in routine analysis of biological samples.PMID 34454386
- detection of lysosomes and lysosome-related organelles, like gut granules in C. elegans intestinal cells.PMID 22226526
- it exhibits strong fluorescence enhancement and is used for staining SDS (sodium dodecyl sulphate) gels.
Biochem/physiol Actions
1 of 1
This Item | |||
|---|---|---|---|
| description Technical grade | description certified by the BSC | description BioReagent, suitable for fluorescence, ≥97.0% (HPLC) | description suitable for microscopy |
| technique(s) titration: suitable | technique(s) microbe id | staining: suitable | technique(s) - | technique(s) - |
| grade technical grade | grade - | grade - | grade - |
| form powder | form powder | form solid | form crystals |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) - | application(s) - |
| solubility methanol: 1 mg/mL | solubility H2O: 1 mg/mL | solubility - | solubility methanol: 0.01 g/10 mL (red to very dark red) |
| storage temp. room temp | storage temp. room temp | storage temp. 2-8°C | storage temp. - |
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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How should n3013 be dissolved and diluted for lipid staining?
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