This DNA ladder can be used with a loading buffer such as G2526:
https://www.sigmaaldrich.com/product/sigma/g2526
P1473
PCR 100 bp Low Ladder
for electrophoresis of PCR fragments
Synonym(s):
100bp maker, 100bp marker for agarose gel electrophoresis, 100pb ladder for agarose gel electrophoresis, DNA ladder, DNA marker, gel ladder, gel marker
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About This Item
NACRES:
NA.25
UNSPSC Code:
41141827
Biological source:
synthetic
Form:
liquid
Storage temp.:
−20°C
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biological source
synthetic
grade
Molecular Biology
form
liquid
usage
75 uses
suitability
suitable for electrophoresis (DNA)
storage temp.
−20°C
Quality Level
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1 of 4
This Item | |||
|---|---|---|---|
| grade for molecular biology | grade for molecular biology | grade - | grade for molecular biology |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
| form liquid | form liquid | form liquid | form liquid |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| usage 75 uses | usage 75 uses | usage sufficient for 50 uses | usage 80 uses |
| suitability suitable for electrophoresis (DNA) | suitability suitable for electrophoresis (DNA) | suitability suitable for electrophoresis (DNA) | suitability suitable for electrophoresis (DNA) |
General description
Sigma′s PCR 100 bp Low Marker contains 10 dsDNA fragments from 100 – 1,000 bases, evenly spaced in 100 bp repeats. Two ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose gel.
The PCR 100 bp low ladder has been specially designed for the size determination of polymerase chain reaction (PCR) generated DNA fragments.
Application
PCR 100 bp Low Ladder has been used:
- in agarose gel electrophoresis as a standard DNA marker to obtain DNA fragments of about 240 bp for polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE) analysis[1]
- to visualize DNA from enzyme digested PCR mixture
- to estimate the molecular weight of the PCR product fragments
Other Notes
Contains 10 bands, 100–1,000 bp in exact 100 bp spaced (ladder) recombinant repeats.
For optimal resolution, the recommended agarose gel concentration is 2.0%.
Sigma′s PCR 100 bp Low Marker is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.
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Articles
Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.
Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.
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Instructions
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What loading buffer/dye is the P1473 DNA ladder compatible with for loading onto a gel.
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