Skip to Content
MilliporeSigma
Get 22% off for Pi Day through March 31.Save Now

PP0201

Glycoprofile™ II, Enzymatic In-Solution N-Deglycosylation Kit

sufficient for 20 samples

Slide 1 of 1
Sign In to View Organizational & Contract Pricing.

Select a Size

1 KIT

$466.00

$466.00

Check Cart for Availability
​
Request a Bulk Order

About This Item

NACRES:
NA.26
UNSPSC Code:
12352202

Key Documents

View All Documentation

Skip To

Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Quality Level

200

storage temp.

2-8°C

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
PP0200PP0540NDEGLY
Glycoprofile™ II, Enzymatic In-Solution N-Deglycosylation Kit sufficient for 20 samples

Sigma-Aldrich

PP0201

Glycoprofile™ II, Enzymatic In-Solution N-Deglycosylation Kit

GlycoProfile™ I, In-Gel Deglycosylation Kit

Sigma-Aldrich

PP0200

GlycoProfile™ I, In-Gel Deglycosylation Kit

GlycoProfile™ β-Elimination Kit Sufficient for 24 samples

Sigma-Aldrich

PP0540

GlycoProfile™ β-Elimination Kit

Native Protein Deglycosylation Kit

Sigma-Aldrich

NDEGLY

Native Protein Deglycosylation Kit

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

room temp

storage temp.

2-8°C

General description

Contains sufficient reagents for a minimum of 20 reactions when the sample size is between one to two mg of a typical glycoprotein.

Application

GlycoProfile II Enzymatic In-Solution N-Deglycosylation Kit was used to study the effects of glycosylation of the N-acetyl-D-galactosamine (GalNAc)-specific lectin found in Schizophyllum commune.[1]
GlycoProfile™ II has been optimized to provide a convenient and reproducible method to remove N-linked glycans from glycoproteins and is compatible with subsequent MALDI-TOF mass spectrometric analysis without interference from any of the reaction components.
Glycoprofile™ II, Enzymatic In-Solution N-Deglycosylation Kit has been used in the deglycosylation of Schizophyllum commune lectin, Arabidopsis mannanase, human umbilical vein endothelial cells (HUVEC) protein lysate and organic solute transporter protein, Ostβ.

Legal Information

GlycoProfile is a trademark of Sigma-Aldrich Co. LLC

Kit Components Only

Product No.
Description
  • 10× Reaction Buffer 1 mL/vial

Kit Components Also Available Separately

Product No.
Description
SDS
  • P7367PNGase F from Elizabethkingia meningoseptica, BioReagent, ≥95% (SDS-PAGE) 50 USDS
  • R7884Ribonuclease B from bovine pancreas, BioReagent, ≥50 Kunitz units/mg protein, ≥80% (SDS-PAGE) .5 mgSDS
  • O9882Octyl β-D-glucopyranoside, BioXtra, ≥98% (GC) 100 mgSDS
  • M31482-Mercaptoethanol, Molecular Biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration) .9 mLSDS

signalword

Danger

Hazard Classifications

Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral

target_organs

Liver,Heart

Storage Class

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk

WGK 3

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
SLCQ1666
SLCQ1665
SLCL8554
SLCJ0236
SLCG4409

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Enzymatic characterization of a glycoside hydrolase family 5 subfamily 7 (GH5\_7) mannanase from Arabidopsis thaliana
Wang Y, et al.
Planta, 239(3), 653-665 (2014)
Christen Y L Yuen et al.
Plant & cell physiology, 58(6), 1103-1117 (2017-04-27)
Members of the protein disulfide isomerase (PDI)-C subfamily are chimeric proteins containing the thioredoxin (Trx) domain of PDIs, and the conserved N- and C-terminal Pfam domains of Erv41p/Erv46p-type cargo receptors. They are unique to plants and chromalveolates. The Arabidopsis genome
Evaluation of soluble junctional adhesion molecule-A as a biomarker of human brain endothelial barrier breakdown
Haarmann A, et al.
PLoS ONE, 5(10), e13568-e13568 (2010)
Podjana Chumkhunthod et al.
Biochimica et biophysica acta, 1760(3), 326-332 (2006-03-02)
An N-acetyl-D-galactosamine (GalNAc)-specific lectin was purified from the edible mushroom, Schizophyllum commune, using affinity chromatography on a porcine stomach mucin (PSM)-Sepharose 4B column. Under reducing and non-reducing conditions, SDS-polyacrylamide gel electrophoresis gave a major band of 31.5 kDa. The Schizophyllum
Purification and characterization of an N-acetyl-D-galactosamine-specific lectin from the edible mushroom Schizophyllum commune
Chumkhunthod P, et al.
Biochim. Biophys. Acta Gen. Subj., 1760(3), 326-332 (2006)
View All Related Papers

Articles

Deglycosylation Kits

Find deglycosylation kits for your research. Review the different features of deglycosylation kits for glycoproteins, enzymatic proteins, and native proteins.

Protocols

Mass Spectrometry of Glycans

Explore mass spectrometry analysis of glycans for glycomic & glycoproteomic neutral & acidic glycan analysis. See a general mass spec glycan analysis procedure.

Global Trade Item Number

SKUGTIN
PP0201-1KT04061836686895

Questions

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service