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SAB4200330

Anti-phospho-CD3ζ (pTyr72) antibody produced in rabbit

IgG fraction of antiserum

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Synonym(s):

Anti-CD247, Anti-CD3H, Anti-CD3Q, Anti-CD3Z, Anti-T cell receptor T3 zeta chain, Anti-T3Z, Anti-TCRK, Anti-Tcrz

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Size/SKUAvailabilityPrice
100 μL
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$610.00

About This Item

NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IP, WB
Citations:
6

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biological source

rabbit

Quality Segment

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

mouse

technique(s)

immunoprecipitation (IP): 2-4 μg using lysates of HEK-293T cells co-expressing mCD3ϲ and constitutively active LCK., western blot: 1:1,000-1:2,000 using lysates of HEK-293T cells co-expressing mCD3ϲ and constitutively active LCK.

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

phosphorylation (pTyr72)

Gene Information

mouse ... Cd247(12503)

General description

T cell antigen receptor ζ chain (CD3ζ), also known as CD247 gene, spanning 88kb of genomic DNA, is mapped to human chromosome 1q24.2.
The T cell antigen receptor-CD3 (TCR-CD3) multimeric complex is composed of TCRαβ, CD3γε, CD3δε and ζ ζ dimers. The CD3γ, CD3δ and CD3ε, each contain one immunoreceptor tyrosine-based activation motif (ITAM), whereas the ζ chain contains three ITAMs.

Immunogen

synthetic phosphopeptide corresponding to residues surrounding Tyr72 of mouse CD3ζ, conjugated to KLH. The corresponding sequence differs by a single amino acid in human.

Application

Anti-phospho-CD3ζ (pTyr72) antibody has been used in
  • immunoblotting
  • immunoprecipitation
  • western blotting

Biochem/physiol Actions

T cell antigen receptor ζchain (CD3ζ) functions as a key signal transduction component of the T cell antigen receptor (TCR) complex. The encoded protein facilitates optimal effector T-cell function by enhancing receptor expression and signaling. Mutation in the gene increases the risk of susceptibility to systemic lupus erythematosus (SLE). Reduced expression of the gene has been observed in T-cells of cancer, lupus and chronic infectious diseases such as leprosy and tuberculosis patients. CD3ζ serves as a potent biomarker for determining progression and severity in patients with type 2 diabetes.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Anti-phospho-CD3ζ (pTyr72) antibody produced in rabbit IgG fraction of antiserum

Sigma-Aldrich

SAB4200330

Anti-phospho-CD3ζ (pTyr72) antibody produced in rabbit

Anti-CD247 antibody produced in rabbit Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution

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Monoclonal Anti-CD3 zeta (PhosphoTyr142) antibody produced in mouse clone EM-54

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Monoclonal Anti-CD3 zeta (PhosphoTyr142) antibody produced in mouse

Anti-CD247 antibody produced in rabbit purified immunoglobulin, buffered aqueous solution

Sigma-Aldrich

SAB1411621

Anti-CD247 antibody produced in rabbit

description

IgG fraction of antiserum

description

Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution

description

clone EM-54

description

purified immunoglobulin, buffered aqueous solution

antibody form

IgG fraction of antiserum

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

Quality Level

200

Quality Level

100

Quality Level

-

Quality Level

100

biological source

rabbit

biological source

rabbit

biological source

mouse

biological source

rabbit

Gene Information

mouse ... Cd247(12503)

Gene Information

human ... CD247(919)

Gene Information

human ... CD247(919)

Gene Information

human ... CD247(919)

shipped in

dry ice

shipped in

wet ice

shipped in

wet ice

shipped in

dry ice

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Storage Class

10 - Combustible liquids

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
071M4755
068M4845V

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