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About This Item
Empirical Formula (Hill Notation):
C32H37N3O2Si
Molecular Weight:
523.74
UNSPSC Code:
12352200
Assay:
≥90% (HPLC)
Form:
powder
Quality Segment
assay
≥90% (HPLC)
form
powder
color
dark blue to black
solubility
DMSO: 2 mg/mL, clear
storage temp.
-10 to -25°C
Biochem/physiol Actions
HMSiR680-Me is a cell-permeable silicon rhodamine (SiR) far-red fluorophore that selectively illuminates acidic organelles, such as lysosomes, in live cells, owing to its unique pH-dependent fluorescence. It is ideal for complex imaging experiments, offering high photostability and minimal impact on cell health or organelle motility. HMSiR680-Me enables multicolor imaging alongside other dyes, with protocols for crosstalk calculation and spectral unmixing to ensure clear and distinct signals. Its utility extends to various applications, including overnight imaging and super-resolution microscopy, making HMSiR680-Me a valuable tool for studying cellular processes and diseases related to lysosomal function. Ex: ~ 680 nm & Em: ~ 695 nm; Detection window ~ 690-830 nm; pKcyclic = 6.2.
Signal Word
Warning
Hazard Codes
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral
Storage Class
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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