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About This Item
Empirical Formula (Hill Notation):
C43H43N3P·Cl · xHCl
Molecular Weight:
668.25 (free base basis)
UNSPSC Code:
12352200
Assay:
≥80% (HPLC)
Form:
powder
Storage condition:
desiccated
Quality Segment
assay
≥80% (HPLC)
form
powder
storage condition
desiccated
color
faint orange to red
solubility
DMSO: 2 mg/mL, clear
shipped in
dry ice
storage temp.
−70°C
Biochem/physiol Actions
Mito-HE is a mitochondria superoxide probe that corresponds to dihydroethidium covalently linked to the mitochondria-targeting triphenylphosphonium (TPP+) cation. Mito-HE is widely used for live cell imaging. Cells are incubated with the probe at a final concentration of 0.3-5 μM for 10-30 min at 37°C before washing. Two different excitation wavelengths are used to distinguish the superoxide-dependent hydroxylation of Mito-HE (385-405 nm) from the nonspecific formation of ethidium (480-520 nm). DMSO stock solutions should be stored at -20°C for no more than 3 weeks. Please refer to Nat Protoc. 2008 3(6):941-7 (PMID 18536642) for experimental setup recommendations.
Disclaimer
Hygroscopic, store with desiccant
Signal Word
Warning
Hazard Codes
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2
Storage Class
11 - Combustible Solids
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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