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SML4361

Coelenterazine 400a

≥97% (HPLC)

Synonym(s):

2,8-Dibenzyl-6-phenyl-7H-imidazo[1,2-a]pyrazin-3-one, 2,8-Dibenzyl-6-phenylimidazo[1,2-a]pyrazin-3(7H)-one, 6-Phenyl-2,8-bis(phenylmethyl)imidazo[1,2-a]pyrazin-3(7H)-one, Bisdeoxycoelenterazine, BlueSyn C, CTZ400a, Coelenterazine HH, DeepBlueC, Dideoxycoelenterazine

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About This Item

Empirical Formula (Hill Notation):
C26H21N3O
CAS Number:
70217-82-2
Molecular Weight:
391.46
UNSPSC Code:
12352200
Assay:
≥97% (HPLC)
Form:
powder
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Quality Segment

assay

≥97% (HPLC)

form

powder

color

white to beige

solubility

DMSO: 2 mg/mL, clear (warmed)

storage temp.

-10 to -25°C

Biochem/physiol Actions

Coelenterazine 400a (CTZ400a), a derivative of coelenterazine, serves as a time-sensitive substrate with moderate bioluminescence quantum yield for Renilla reniformis luciferase (RLuc) and its variants. However, it exhibits poor oxidation efficiency with Gaussia luciferase (GLuc). Due to its emission maximum of approximately 400 nm, CTZ400a is the preferred substrate for Bioluminescence Resonance Energy Transfer (BRET) as it minimally interferes with GFP emission. This property enhances signal resolution, making Coelenterazine-400a a valuable tool for real-time studies of protein-protein interactions in live cells. Additionally, Coelenterazine400a is an efficient and suitable substrate for the luminescence reaction of nanoKAZ.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Codes

H302

Precautionary Statements

P264 - P270 - P301 + P312 - P501

Hazard Classifications

Acute Tox. 4 Oral

Storage Class

11 - Combustible Solids

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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