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SML4412

BMOE

≥98% (HPLC)

Synonym(s):

1,2-Bismaleimidoethane; Bis-maleimidoethane; Ethylenebismaleimide; 1,1′-(1,2-Ethanediyl)bis[1H-pyrrole-2,5-dione], BMOE, Short-arm Cross-linker, Cross-linker BMOE

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About This Item

Empirical Formula (Hill Notation):
C10H8N2O4
CAS Number:
5132-30-9
Molecular Weight:
220.18
UNSPSC Code:
12352200
Assay:
≥98% (HPLC)
Form:
powder
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Quality Segment

assay

≥98% (HPLC)

form

powder

color

white to beige

solubility

DMSO: 2 mg/mL, clear

storage temp.

-10 to -25°C

Biochem/physiol Actions

BMOE is a membrane-permeable, rigid, irreversible homo-bifunctional cross-linker with a short spacer arm (~8 Å) that forms a stable covalent thioether bond at pH 6.5–7.5, inducing conformational changes in proteins. Bismaleimidoethane effectively targets bis-cysteines within a 6.3–10.5 Å distance, enabling near-stoichiometric cross-linking and facilitating the study of protein-protein interactions and complex architecture in native cellular environments. BMOE is particularly useful for probing the quaternary structure of proteins, exploring oligomerization, and characterizing disulfide bond formation in intact cells.

Storage Class

11 - Combustible Solids

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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