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MilliporeSigma

SML4438

5-TAMRA-Alkyne

≥98% (HPLC)

Synonym(s):

5-Carboxytetramethylrhodamine, 9-[2-Carboxy-4-[(2-propyn-1-ylamino)carbonyl]phenyl]-3,6-bis(dimethylamino)-xanthylium inner salt, Propargylamide, TAMRA-Alkyne isomer 5

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About This Item

Empirical Formula (Hill Notation):
C28H25N3O4
CAS Number:
Molecular Weight:
467.52
UNSPSC Code:
12352200
Assay:
≥98% (HPLC)
Form:
powder
Storage condition:
desiccated, protect from light
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Quality Segment

assay

≥98% (HPLC)

form

powder

storage condition

desiccated, protect from light

color

red to very dark purple

solubility

DMSO: 2 mg/mL, clear

storage temp.

-10 to -25°C

Biochem/physiol Actions

TAMRA-Alkyne is a cell-permeable, and photostable bioorthogonal probe for the high-fidelity covalent labeling of azide-functionalized biomolecules. This tool leverages the bright, orange-red fluorescence (λex/λem ~546 nm/579 nm) of an isomerically pure 5-TAMRA core and the specificity of copper-catalyzed click chemistry. It is specifically designed as a compact, fluorescent alkyne for situations where larger, PEGylated probes might introduce steric interference or are otherwise undesirable, offering a minimal steric footprint for probing constrained molecular environments. While TAMRA-alkyne’s hydrophobicity necessitates rigorous protocols to manage non-specific binding, it remains a valuable tool for fixed-cell imaging where a small tag is paramount for achieving high-resolution visualization.

Disclaimer

Hygroscopic, Store with desiccant


Storage Class

11 - Combustible Solids

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable



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