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About This Item
Empirical Formula (Hill Notation):
C28H28N6O4
CAS Number:
Molecular Weight:
512.56
UNSPSC Code:
12352200
Assay:
≥98% (HPLC)
Form:
powder
Storage condition:
desiccated, protect from light
Quality Segment
assay
≥98% (HPLC)
form
powder
storage condition
desiccated, protect from light
color
red to very dark purple
solubility
DMSO: 2 mg/mL, clear
storage temp.
-10 to -25°C
Biochem/physiol Actions
5-TAMRA Azide is a photostable, azide-functionalized carboxy-tetramethylrhodamine reporter that enables specific covalent labeling of alkyne-tagged biomolecules via bioorthogonal click chemistry. Its utility spans both copper-catalyzed (CuAAC) and copper-free strain-promoted (SPAAC) cycloadditions, facilitating applications from fixed-sample proteomics to real-time live-cell imaging. The probe leverages a bright TAMRA fluorophore (λex/λem ≈ 546/579 nm; ε ≈ 91,000 M⁻¹cm⁻¹; Φ ≈ 0.4) that maintains stable fluorescence from pH 4–9, ensuring robust quantification in fluorescence microscopy, flow cytometry, and in-gel scanning. Its pivotal advantage is the fusion of high-fidelity bioorthogonal reactivity with an orange-red spectral profile ideal for multiplexed analysis, minimizing interference from cellular autofluorescence and crosstalk with common blue and green fluorescent channels.
Disclaimer
Hygroscopic, Store with desiccant
Signal Word
Warning
Hazard Codes
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral
Storage Class
11 - Combustible Solids
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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