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Trypsin solution from porcine pancreas

Name: Trypsin solution from porcine pancreas
Brand: SIGMA
Price: 16.5 GBP

sterile-filtered, BioReagent, suitable for cell culture, 25 g porcine trypsin per liter in 0.9% sodium chloride

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Size/SKU	Availability	Price
20 mL	Please contact Customer Service for Availability	£16.50
100 mL	Please contact Customer Service for Availability	£18.80

About This Item

CAS Number:

9002-07-7

EC Number:

3.4.21.4 (BRENDA, IUBMB)

UNSPSC Code:

12352204

NACRES:

NA.75

MDL number:

MFCD00082094

Biological source:

Porcine

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Properties

biological source

Porcine

Quality Segment

500

sterility

sterile-filtered

product line

BioReagent

form

solution

mol wt

23.4 kDa

technique(s)

cell culture | mammalian: suitable

pH

7.6

shipped in

dry ice

storage temp.

−20°C

Description

Application

For trypsin digestion of peptides, use a ratio of about 1:100 to 1:20 for trypsin:peptide. The typical use for this product is in removing adherent cells from a culture surface. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture. Trypsins have also been used for the re-suspension of cells during cell culture, in proteomics research for digestion of proteins and in various in-gel digestions. Additional applications include assessing crystallization by membrane-based techniques and in a study to determine that protein folding rates and yields can be limited by the presence of kinetic traps.

Biochem/physiol Actions

Trypsin cleaves peptides on the C-terminal side of lysine and arginine residues. The rate of hydrolysis of this reaction is slowed if an acidic residue is on either side of the cleavage site and hydrolysis is stopped if a proline residue is on the carboxyl side of the cleavage site. The optimal pH for trypsin activity is 7-9. Trypsin can also act to cleave ester and amide linkages of synthetic derivatives of amino acids. EDTA is added to trypsin solutions as a chelating agent that neutralizes calcium and magnesium ions that obscure the peptide bonds on which trypsin acts. Removing these ions increases the enzymatic activity.

Serine protease inhibitors, including DFP, TLCK, APMSF, AEBSEF, and aprotinin, amongst others, will inhibit Trypsin.

Preparation Note

This is 25 g/L porcine trypsin solution in 0.9% sodium chloride.

Other Notes

One BAEE unit will produce a A₂₅₃ of 0.001 per minute at pH 7.6 at 25°C using BAEE as a substrate.

Disclaimer

This product should be stored frozen at -20°C.

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This Item	T4424	T4674	T4799
Sigma-Aldrich T4549 Trypsin solution from porcine pancreas	Sigma-Aldrich T4424 Trypsin solution from porcine pancreas	Sigma-Aldrich T4674 Trypsin solution from porcine pancreas	Sigma-Aldrich T4799 Trypsin from porcine pancreas
description sterile-filtered, BioReagent, suitable for cell culture, 25 g porcine trypsin per liter in 0.9% sodium chloride	description 1 ×, sterile-filtered, BioReagent, suitable for cell culture, 2.5 g porcine trypsin per liter in Hanks′ Balanced Salt Solution with phenol red	description sterile-filtered, BioReagent, suitable for cell culture, 25 g porcine trypsin per liter in Hanks′ Balanced Salt Solution with phenol red	description lyophilized powder, BioReagent, suitable for cell culture, 1,000-2,000 BAEE units/mg solid
biological source Porcine	biological source Porcine pancreas	biological source Porcine pancreas	biological source Porcine pancreas
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) single cell analysis: suitable, cell culture \| mammalian: suitable
form solution	form solution	form solution	form lyophilized powder
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
shipped in dry ice	shipped in dry ice	shipped in dry ice	shipped in ambient
pH 7.6	pH 7.0-7.6	pH 7.6	pH 7.6

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Pictograms

GHS08,GHS07

Signal Word

Danger

Hazard Codes

H315,H319,H334,H335

Precautionary Statements

P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class

10 - Combustible liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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