Ultra pure BST-RT Polymerase

BST-RT Polymerase is an engineered Bst enzyme with robust reverse transcriptase (RT) activity, making it ideal for use in loop-mediated isothermal amplification (LAMP) or RT-LAMP assays. This feature eliminates the necessity of a secondary RT enzyme when targeting amplification from RNA samples, simplifying reaction setup. BST-RT works optimally at 65°C and can be used for real time reaction monitoring with a fluorescent dye or in endpoint detection assays. BST-RT polymerase is tolerant to potential reaction inhibitors such as ethanol, extraction buffers, and saliva. This enzyme is also compatible with workflows that incorporate dUTP for elimination of reaction carryover contaminants.

Loop-mediated isothermal amplification (LAMP) has emerged as a key alternative to polymerase chain reaction (PCR)-based techniques for amplifying nucleic acids. While conventional PCR employs a thermal cycler for repetitive cycling temperatures when amplifying, isothermal amplification techniques occur at a single and fixed temperature, allowing the use of simple,portable, and more robust instruments for fast and exponential amplification.

In solution.

One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 65°C. Order primers for LAMP using our online configurator. For assistance with primer design prior to purchase, please use the online design tool from our partner.