V3003
Monoclonal Anti-Vascular Endothelial Growth Factor Receptor-2 (KDR) antibody produced in mouse
clone 260.4, KDR-2, ascites fluid
Synonym(s):
Monoclonal Anti-Vascular Endothelial Growth Factor Receptor-2 antibody produced in mouse, Anti-KDR, Anti-VEGFR-2
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0.2 ML
$637.00
$637.00
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About This Item
NACRES:
NA.41
UNSPSC Code:
51111800
Conjugate:
unconjugated
Clone:
260.4, KDR-2, monoclonal
Application:
immunohistochemistry (frozen sections)
indirect ELISA
western blot
indirect ELISA
western blot
Species reactivity:
human
Citations:
13
Technique(s):
immunohistochemistry (frozen sections): suitable
indirect ELISA: suitable
western blot: 1:500 using insect cell culture supernatant expressing human extracellular recombinant VEGFR or VEGF Receptor-2 (KDR, FLK-1)/FC Chimera human, recombinant
indirect ELISA: suitable
western blot: 1:500 using insect cell culture supernatant expressing human extracellular recombinant VEGFR or VEGF Receptor-2 (KDR, FLK-1)/FC Chimera human, recombinant
Uniprot accession no.:
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Product Name
Monoclonal Anti-Vascular Endothelial Growth Factor Receptor-2 (KDR) antibody produced in mouse, clone 260.4, KDR-2, ascites fluid
biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
260.4, KDR-2, monoclonal
contains
15 mM sodium azide
species reactivity
human
technique(s)
immunohistochemistry (frozen sections): suitable
indirect ELISA: suitable
western blot: 1:500 using insect cell culture supernatant expressing human extracellular recombinant VEGFR or VEGF Receptor-2 (KDR, FLK-1)/FC Chimera human, recombinant
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... KDR(3791)
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1 of 4
This Item | V9134 | SAB1404004 | ABS553 |
|---|---|---|---|
| biological source mouse | biological source mouse | biological source mouse | biological source rabbit |
| clone 260.4, KDR-2, monoclonal | clone KDR-1, monoclonal | clone 4A2, monoclonal | clone polyclonal |
| Gene Information human ... KDR(3791) | Gene Information human ... KDR(3791) | Gene Information human ... KDR(3791) | Gene Information human ... KDR(3791) |
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate - |
| antibody form ascites fluid | antibody form ascites fluid | antibody form purified immunoglobulin | antibody form affinity isolated antibody |
| species reactivity human | species reactivity human | species reactivity human | species reactivity human, mouse |
Application
Biochem/physiol Actions
Reacts specifically with human VEGF receptor-2. This clone has also been termed 260.4. It does not recognize Flt1 (VEGFR-1), Flt4 (VEGFR-3), or platelet-derived growth factor receptor (PDGF-R).
Vascular Endothelial Growth Factor Receptor-2 (VEGF R2) have high affinity for vascular endothelial growth factor-A (VEGF-A), hence facilitates most of the endothelial growth and survival signals from VEGF-A. It also plays a pivotal role in vascular development, vascular permeability, embryonic hematopoiesis and regulation of angiogenesis.
Vascular endothelial growth factor receptor 2 (VEGFR2) or kinase insert domain receptor (KDR) is a VEGF receptor, which stimulates its mitogenic activity. It is crucial for vascular endothelial cell development. VEGFR2 expression is elevated in invasive breast cancer, hemangiomas and angiosarcomas. KDR expressing cells show striking changes in cell morphology, actin reorganization and membrane ruffling, chemotaxis, and mitogenicity in response to VEGF. Antibodies that react specifically with the VEGF receptor are useful for the study of the specific differential tissue expression and intracellular localization of the receptor in normal and neoplastic tissue.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Monoclonal Anti-Vascular Endothelial Growth Factor Receptor-2 (mouse IgG1 isotype) is derived from the KDR-2 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Vascular endothelial growth factor receptor 2 (VEGFR2) is a type V receptor tyrosine kinase, also known as kinase insert domain receptor (KDR) or fetal liver kinase 1 (Flk-1). It a glycosylated protein with an immunoglobulin (Ig)-like domains, a transmembrane domain and a tyrosine kinase domain. The KDR gene is mapped to human chromosome 4q12 and is widely expressed in all vessel-derived endothelial cells and hematopoietic stem cells.
The KDR receptor (VEGFRa), defining marker of hematopoietic stem cells (HSCs), distinguishes pluripotent HSCs from lineage-committed progenitor HSCs.
Immunogen
purified recombinant human extracellular VEGF receptor-2 (KDR).
Physical form
The product is provided as ascites fluid with 15 mM sodium azide as a preservative.
Preparation Note
For continuous use, store at 2-8 °C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
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Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Irawan Satriotomo et al.
Experimental neurology, 237(1), 103-115 (2012-06-19)
Acute intermittent hypoxia (AIH) initiates plasticity in respiratory motor control, including phrenic long term facilitation (pLTF). Since pLTF is enhanced by preconditioning with repetitive exposure to AIH (rAIH), we hypothesized that a rAIH protocol consisting of 3 AIH exposures per
S Ogawa et al.
The Journal of biological chemistry, 273(47), 31273-31282 (1998-11-13)
Vascular endothelial growth factor (VEGF) mediates endothelial cell proliferation, angiogenesis, and vascular permeability via the endothelial cell receptors, KDR/Flk-1 and Flt-1. Recently, a gene encoding a polypeptide with about 25% amino acid identity to mammalian VEGF was identified in the
Nikola Holtkamp et al.
Neuro-oncology, 9(3), 291-297 (2007-05-17)
A subset of glioblastomas (GBMs) carry gene amplifications on chromosomal segment 4q12. To characterize this amplicon in detail, we analyzed a set of 100 samples consisting of 65 GBMs, 10 WHO grade III astrocytomas, 12 oligodendrogliomas, and 13 glioma cell
Markku Miettinen et al.
The American journal of surgical pathology, 36(4), 629-639 (2012-02-09)
Vascular endothelial growth factor receptor 2 (VEGFR2) is a primary responder to vascular endothelial growth factor signal and thereby regulates endothelial migration and proliferation. This receptor is expressed in endothelial cells and in some vascular tumors, but many reports also
I Satriotomo et al.
Neuroscience, 322, 479-488 (2016-03-06)
Repetitive acute intermittent hypoxia (rAIH) increases growth/trophic factor expression in respiratory motor neurons, thereby eliciting spinal respiratory motor plasticity and/or neuroprotection. Here we demonstrate that rAIH effects are not unique to respiratory motor neurons, but are also expressed in non-respiratory
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