ZytoLight^® SPEC MDM2/CEN 12 Dual Color Probe

The ZytoLight^® SPEC MDM2/CEN 12 Dual Color Probe (PL18) is a direct‑labeled dual‑color fluorescence in situ hybridization (FISH) probe for detecting the human MDM2 gene region together with alpha‑satellite sequences of chromosome 12 in formalin‑fixed, paraffin‑embedded specimens.It combines differently labeled probe fractions targeting the MDM2 locus and chromosome 12 centromeric sequences to support visualization of copy number changes at these regions by FISH on tissue sections.

Principle:

This probe is intended for use in fluorescence in situ hybridization (FISH) procedures on formalin-fixed, paraffin-embedded tissue specimens. It is designed to support the analysis of genetic features and should be used alongside other relevant information and methods for comprehensive evaluation.

Components:Probe Composition:

• ZyGreen: Labeled polynucleotides (excitation 503 nm/emission 528 nm) targeting sequences in 12q15 (harboring the MDM2 gene).
• ZyOrange: Labeled polynucleotides (excitation 547 nm/emission 572 nm) targeting sequences in 12p11.1-q11 (specific for the alpha satellite centromeric region D12Z3 of chromosome 12).
• Hybridization Buffer: Formamide-based hybridization buffer.Related Products: Intended for use with the ZytoLight FISH-Tissue Implementation Kit (Prod. No. Z-2028-5/-20).

This probe is intended for use in fluorescence in situ hybridization (FISH) procedures on formalin-fixed, paraffin-embedded tissue specimens. It is designed to support the analysis of genetic features and should be used alongside other relevant information and methods for comprehensive evaluation.

For the qualitative detection of human MDM2 gene amplifications and alpha satellites of chromosome 12 by fluorescence in situ hybridization (FISH)

The performance of the probe has been validated for detecting amplifications of the MDM2 gene locus. In normal interphase cells, two green signals (representing the MDM2 gene region) and two orange signals (representing the centromeric region of chromosome 12) are expected. In cases of amplification, multiple copies of the green signal or clusters will be observed.

ZytoLight is a registered trademark of ZytoVision GmbH in Germany