ZytoLight^® SPEC RET Dual Color Break Apart Probe

The ZytoLight SPEC RET Dual Color Break Apart Probe (PL105) is a direct-labeled FISH probe designed for the qualitative detection of translocations involving the human RET gene at 10q11.21 in formalin-fixed, paraffin-embedded (FFPE) specimens. It is intended to be used with the ZytoLight FISH-Tissue Implementation Kit.

Components:

ZytoLight® SPEC RET Dual Color Break Apart Probe comprises:

• ZyGreen labeled polynucleotides (excitation 503 nm/emission 528 nm) targeting sequences distal to the RET breakpoint region on chromosome 10 (10q11.21).
• ZyOrange labeled polynucleotides (excitation 547 nm/emission 572 nm) targeting sequences proximal to the RET breakpoint region.
• Formamide-based hybridization buffer.

Principle:Fluorescence in situ hybridization (FISH) uses direct-labeled probes that co-denature with target DNA in pretreated FFPE sections (10 µl/slide, sealed), hybridize overnight (75°C/10 min denaturation, 37°C hybridization), then undergo stringent washing and DAPI counterstaining for visualization via fluorescence microscopy with color-specific filters per ZytoLight FISH-Tissue Implementation Kit.

For the qualitative detection of translocations involving the human RET gene at 10q11.21 by fluorescence in situ hybridization (FISH)

ZytoLight^® SPEC RET Dual Color Break Apart Probe is designed for the qualitative assessment of rearrangements involving the RET gene locus in formalin‑fixed, paraffin‑embedded human tissue sections using fluorescence in situ hybridization (FISH). It is intended for use by trained personnel in appropriately equipped laboratories as part of broader histopathological and molecular investigations, with results to be interpreted in conjunction with additional laboratory findings and relevant clinical information.

In a normal interphase nucleus, two green signals (for RET) and two orange signals (for the RET breakpoint region) are expected. In cases of translocation, separate signals will be observed. Signal patterns should be interpreted carefully to identify potential genetic rearrangements.

ZytoLight is a registered trademark of ZytoVision GmbH in Germany