1.25080
Fractogel® EMD DMAE (M)
MiniChrom Fractogel® DMAE (M), volume 5 mL, prepacked column
Sinónimos:
MiniChrom Column Fractogel® DMAE (M), 5 ml
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ligand
diethyl-(2-hydroxypropyl)aminoethyl
material
polypropylene housing
product line
Fractogel®
form
prepacked column
parameter
≤8 bar pressure
~170 cm/hr max. flow rate
column I.D. × L
8 mm × 100 mm
volume
5 mL
matrix active group
Methacrylate
mean particle size
40-90 μm
capacity
~100 mg, BSA binding capacity
application(s)
gene therapy
recombinant protein
vaccine development
separation technique
anion exchange
storage temp.
15-25°C
1 of 4
Este artículo | 1.25071 | 1.25070 | 1.25082 |
|---|---|---|---|
| volume 5 mL | volume 5 mL | volume 5 mL | volume 5 mL |
| ligand diethyl-(2-hydroxypropyl)aminoethyl | ligand (Trimethylammoniumethyl) | ligand (Trimethylammoniumethyl) | ligand carboxymethyl |
| capacity ~100 mg, BSA binding capacity | capacity ~100 mg, BSA binding capacity | capacity ~180 mg, BSA binding capacity | capacity ~100 mL, resin binding capacity |
| form prepacked column | form prepacked column | form prepacked column | form prepacked column |
| material polypropylene housing | material polypropylene housing | material polypropylene housing | material polypropylene housing |
| separation technique anion exchange | separation technique anion exchange | separation technique anion exchange | separation technique anion exchange |
General description
Features and Benefits
- Excellent binding to large viruses and plasmid DNA
- Homogenous binding with high selectivity and purity
- Lower elution volumes for the highest purity levels
- Compatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution
Due to the titration behavior, the ion exchange capacity can be used from pH 2 to pH 9.5. The separation of proteins is based on reversible electrostatic interactions between the negatively charged regions of the proteins′ surface and the support. Proteins are retained efficiently on Fractogel® EMD DEAE when the pH of the buffer is about 1 unit above their isoelectric points (pl).
The strength of the binding depends on the following:
- the buffer system
- pH value of the buffer which determines the surface charge of the protein
- the degree of the ionization of the functional groups of the exchanger
- the concentration of the counter ions
- the charge density on the support (protein binding capacity)
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