70794
BugBuster® GST-Bind Purification Kit
Affinity purification of GST fusion proteins
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manufacturer/tradename
Novagen®
storage condition
OK to freeze
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Este artículo | 70922 | 70584-M | 70751 |
|---|---|---|---|
| manufacturer/tradename Novagen® | manufacturer/tradename Novagen® | manufacturer/tradename Novagen® | manufacturer/tradename Novagen® |
| storage condition OK to freeze | storage condition OK to freeze, avoid repeated freeze/thaw cycles | storage condition OK to freeze, avoid repeated freeze/thaw cycles | storage condition OK to freeze |
General description
Other Notes
•10,000 UBenzonase Nuclease, purity >90%
•10 mlGST•Bind Resin
•pkg/4Chromatography Columns
•2 x 100 ml10X GST Bind/Wash Buffer
•40 ml10X Glutathione Reconstitution Buffer
•1 gGlutathione, Reduced
Legal Information
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Warning
hcodes
Hazard Classifications
Flam. Liq. 3
Clase de almacenamiento
3 - Flammable liquids
flash_point_f
96.8 °F
flash_point_c
36 °C
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Contenido relacionado
Traditionally, protein purification from E. coli consists of four distinct phases: harvest, bacterial cell lysis, lysate clarification and protein purification. Bacterial lysis typically requires several time-consuming, hands-on steps, such as freeze/thaw cycles and sonication. These harsh lysis techniques may negatively impact protein quality and contribute to sample-to-sample variability. To maintain protein activity and integrity, detergent-based lysis buffers are routinely used to avoid mechanical protein extraction methods. Regardless of the lysis method used, centrifugation is traditionally required to pellet unwanted cell debris and permit recovery of the clarified lysate. The final step, purification, is frequently performed using affinity media specific for expressed epitope tags. Agarose-based media have typically been used, either as a slurry in microcentrifuge tubes or packed into gravity-driven or spin columns. While easier to manipulate, columns are greatly affected by lysate consistency and carryover of cell debris, which can lead to clogging of the column frits.
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