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UNSPSC Code:
12352203
eCl@ss:
32160702
Servicio técnico
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Permítanos ayudarleServicio técnico
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Permítanos ayudarleconjugate
unconjugated
Quality Level
antibody form
affinity purified immunoglobulin
clone
polyclonal
purified by
(ImmunoAffinity Purified)
species reactivity
rabbit, rat, mouse
technique(s)
immunohistochemistry: suitable
western blot: suitable
UniProt accession no.
target post-translational modification
unmodified
General description
FUNCTION: SwissProt: P20783 # Seems to promotes the survival of visceral and proprioceptive sensory neurons.
SIZE: 257 amino acids; 29355 Da
SUBCELLULAR LOCATION: Secreted.
TISSUE SPECIFICITY: Brain and peripheral tissues.
SIMILARITY: SwissProt: P20783 ## Belongs to the NGF-beta family.
SIZE: 257 amino acids; 29355 Da
SUBCELLULAR LOCATION: Secreted.
TISSUE SPECIFICITY: Brain and peripheral tissues.
SIMILARITY: SwissProt: P20783 ## Belongs to the NGF-beta family.
Purified peptide from the pro domain of the human NT-3 protein (Accession number P20783).
Application
Western blot on recombinant mouse proNT-3
Immunohistochemistry on mouse brain sections.
Dilutions should be made using a carrier protein such as BSA (1-3%).
Optimal working dilutions must be determined by the end user.
Immunohistochemistry on mouse brain sections.
Dilutions should be made using a carrier protein such as BSA (1-3%).
Optimal working dilutions must be determined by the end user.
Biochem/physiol Actions
Recognizes proNT-3 (pro Neurotrophin-3).
Physical form
Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, and 0.05% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
Preparation Note
Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Other Notes
Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Clase de almacenamiento
10 - Combustible liquids
Certificados de análisis (COA)
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