Catalog number F7524 is not tested for tetracycline, so we cannot guarantee that the product is tetracycline free. Product F2442 is regularly tested for tetracycline and the results shown in a lot-specific certificate of analysis. The website page for this product is linked here: https://www.sigmaaldrich.com/catalog/product/sigma/f2442?lang=fr®ion=CH
F7524
Suero fetal bovino
non-USA origin, sterile-filtered, suitable for cell culture
Fetal Bovine Serum
1 of 8 reviewers received a sample product or took part in a promotion
Sinónimos:
FBS, FCS, Suero, sueros
Iniciar sesión para ver los posibles precios especiales de su organización
Seleccione un Tamaño
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Acerca de este artículo
Biological source:
bovine fetus
Origin:
non-USA origin
Sterility:
sterile-filtered
Shipped in:
dry ice
Saltar a
Servicio técnico
¿Necesita ayuda? Nuestro equipo de científicos experimentados está aquí para ayudarle.
Permítanos ayudarlebiological source
bovine fetus
Quality Level
sterility
sterile-filtered
product line
FBS Classic
composition
hemoglobin, ≤25 mg/dL
origin
non-USA origin
availability
not available in Canada, USA
technique(s)
cell culture | mammalian: suitable
impurities
≤10 EU/mL endotoxin
shipped in
dry ice
storage temp.
−20°C
¿Está buscando productos similares? Visita Guía de comparación de productos
Categorías relacionadas
1 of 4
Este artículo | 12003C | F6765 | F9665 |
|---|---|---|---|
| origin non-USA origin | origin Australia origin | origin USA origin | origin non-USA origin |
| sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered |
| biological source bovine fetus | biological source bovine fetus | biological source bovine fetus | biological source bovine fetus |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| composition hemoglobin, ≤25 mg/dL | composition Hemoglobin, ≤25 mg/dL | composition Bovine IgG, ≤1 mg/mL , Hemoglobin, ≤20 mg/dL | composition Hemoglobin, ≤25 mg/dL |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
Application
El suero bovino fetal se ha utilizado como suplemento en medios de cultivo celular como el medio Roswell Park Memorial Institute (RPMI-1640) y el medio Eagle modificado de Dulbecco (DMEM)[1] para propiciar el crecimiento de una variedad de líneas celulares.
Biochem/physiol Actions
El suero bovino fetal (FBS) sirve como un suplemento de medios de cultivo celular comúnmente utilizados para cultivos de células, tejidos y órganos in vitro y ex vivo. El FBS sigue siendo un complemento popular para los medios porque aporta una amplia gama de funciones al cultivo celular. El FBS aporta nutrientes, factores de crecimiento y de unión, y protege a las células del daño oxidativo y la apoptosis mediante mecanismos que son difíciles de reproducir en los sistemas de medios sin suero (SFM). Proporciona muchos factores no definidos de promoción del crecimiento y de mejora de la supervivencia de las células en cultivo. Procedente de sangre coagulada, es el suplemento no definido más ampliamente utilizado en cultivos de células eucariotas, en especial de mamíferos. El FBS suele utilizarse con los medios clásicos que se desarrollaron en las décadas de 1950 y 1960, entre ellos muchos medios populares como el medio mínimo esencial (MEM); los medios de Eagle modificados por Dulbecco (DME, DMEM); el medio de Eagle modificado por Iscove (IMDM); el medio M199 y del Instituto Roswell Park Memorial, RPMI-1640.
Features and Benefits
El F7524 es el mismo gran producto que el producto S0115 de Biochrom GmbH.
Analysis Note
- Analizada la presencia de endotoxinas y hemoglobina
- Se ha estudiado la presencia de bacterias, virus y micoplasmas
- Filtrado triple con membrana de 0,1 micra bajo condiciones asépticas
Legal Information
No disponible en EE.UU.ni en Canadá. Compruebe con su proveedor local la disponibilidad y las restricciones a la importación de su país.
Related product
Referencia del producto
Descripción
Precios
Clase de almacenamiento
10 - Combustible liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
Elija entre una de las versiones más recientes:
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Hans F N Kvitvang et al.
Journal of chromatography. A, 1370, 70-79 (2014-12-03)
Metabolic profiling has become an important tool in biological research, and the chromatographic separation of metabolites coupled with mass spectrometric detection is the most frequently used approach for such studies. The establishment of robust chromatographic methods for comprehensive coverage of
Elisabeth F P Peterse et al.
BMC cancer, 16, 475-475 (2016-07-16)
Chondrosarcoma is a malignant cartilage forming bone tumour for which no effective systemic treatment is available. Previous studies illustrate the need for a better understanding of the role of the IGF pathway in chondrosarcoma to determine if it can be
Carlo E A Jochems et al.
Alternatives to laboratory animals : ATLA, 30(2), 219-227 (2002-04-25)
Fetal bovine serum (FBS) is a common component of animal cell culture media. It is harvested from bovine fetuses taken from pregnant cows during slaughter. FBS is commonly harvested by means of a cardiac puncture without any form of anaesthesia.
Michelle J Nyhan et al.
BMC cancer, 16, 101-101 (2016-02-18)
Successful treatment of oesophageal cancer is hampered by recurrent drug resistant disease. We have previously demonstrated the importance of apoptosis and autophagy for the recovery of oesophageal cancer cells following drug treatment. When apoptosis (with autophagy) is induced, these cells
Jiří Voller et al.
Bioorganic chemistry, 90, 103005-103005 (2019-07-05)
Rho-associated serine/threonine kinases (ROCKs) are principal regulators of the actin cytoskeleton that regulate the contractility, shape, motility, and invasion of cells. We explored the relationships between structure and anti-ROCK2 activity in a group of purine derivatives substituted at the C6
Artículos
Review the basics of using fetal bovine (or calf) serum for cell culture and how sourcing and regulations affect the price of sera for cell-based assays.
Contenido relacionado
Explore nuestra gama de suero bovino fetal (FBS) y determine el FBS adecuado para sus requisitos y presupuesto de laboratorio. Solicite una muestra de suero.
-
Does the F7524 Fetal Bovine Serum product contain Tetracycline?
1 answer-
Helpful?
-
-
When aliquioting FBS, is it best to cool them to 4ºC first or directly at -80ºC
1 answer-
The serum can be aliquoted into smaller, single-use portions and refrozen directly.
Helpful?
-
-
Hello, I have a question regarding FBS (F7524), for my experiment it is essential there is no dopamine or serotonine in my medium. Is it known wether these substances are present in FBS?
1 answer-
This product has not undergone testing to determine the presence of dopamine or serotonin.
Helpful?
-
-
Could the presence of insoluble precipitates when thawing the serum indicate contamination?
1 answer-
Thawed serum may contain precipitates and flocculants due to various reasons, such as residual fibrinogen converting to fibrin upon thawing. Centrifugation at 400 xg can remove excess fibrin, while filtering serum is not recommended to avoid protein depletion and clogged filters.
Additional points to consider:
•Cloudiness in human serum products is common and indicates the presence of lipids. Clear, lipid-free human serum products are not available.
•Microscopic serum precipitates may resemble yeast contamination but are actually lipid aggregates that can be dissolved by lipase. They do not show growth in culture or gram staining and can be removed through centrifugation or filtration.
•Gel layer or clumps in serum are caused by poor or overheated inactivation or improper mixing of FBS during the Heat inactivation process. Serum with these characteristics should not be used.
•To minimize denaturation of serum lipoproteins and turbidity, it is recommended to prepare single-use aliquots and avoid repeated freeze-thaw cycles.Helpful?
-
-
Is this product F7524 heat-inactivated? Should it be prior to use?
1 answer-
This product has not been heat inactivated. For most cell culture applications heat inactivation is not necessary. If the application requires inactivation of any heat labile serum components, such as compliment or cytokines, the product may be heat inactivated in the lab. Alternatively, a heat inactivated version of this product is also available. See product F9665.
To heat inactivate this product:
1. Allow serum containers to come to room temperature. Swirl bottles of serum immediately before adding to the water bath.
2. A water-filled control bottles (T1) should be placed in a 56 °C water bath. Place the containers in the water bath up to the serum line. Do not completely submerge the containers. When the temperature of T1 reaches 56 °C, start the timer set for 30 minutes. Do not allow temperatures to exceed 56 °C. Higher temperatures will denature the proteins, gel the serum or alter the performance of the serum.
3. Gently swirl the bottles every 5 to 10 minutes while in the water bath and check the temperature in the control bottle. It is not uncommon for plastic containers to bow slightly after heat treatment. This is not harmful to the serum.
4. At the end of 30 minutes remove the serum bottles from the water bath. Aliquot the serum into smaller volumes and freeze. Avoid repeated thaw-freeze cycles.Helpful?
-
-
Bonjour, Quelle est la composition et la concentration du sérum référencé F7524 en Calcium ? Cordialement
1 answer-
Calcium levels are not determined for this product. The serum is a natural product that will contain calcium at physiological levels.
Helpful?
-
-
Welches fetale Kälbserum wurde für die Kryokonservierung von Mausembryonen oder Eiestockmäus verwendet?
1 answer-
Unfortunately, a fetal calf serum that has been specifically tested for cryopreservation of mouse embryos or ovarian mice is not currently offered. Please see the link below to review Product ES009-M, Fetal Bovine Serum. This product is tested for expansion and cytotoxicity of mouse embryonic stem cells and may be of interest:
https://www.sigmaaldrich.com/product/mm/es009mHelpful?
-
-
How can we heat inactivate the fetal bovine serum cat: F7524? For how long?
1 answer-
All heat inactivated serum products are treated at 56 deg. C for 30 minutes. This process may be applied to manually heat inactivate serum on a per bottle basis using a water bath. When immersing bottles do not allow water to come in contact with the cap/closure.
Helpful?
-
-
Is Feta bovine serum F7524-500ML heat inactivated?
1 answer-
This product is not heat-inactivated.
Helpful?
-
-
What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
-
Active Filters
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico