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TMS-010

Alcian-Blue Staining Solution

Synonym(e):

Alcian blue 8G, Alcian blue PAS stain, Alcian blue stain

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Größe/SKUVerfügbarkeitPreis
100 mL
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€ 130,00

Über diesen Artikel

NACRES:
NA.75
UNSPSC Code:
12352207

€ 130,00

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Quality Segment

technique(s)

cell based assay: suitable, cell culture | stem cell: suitable, cell differentiation: suitable

General description

Alcian blues, are a family of polyvalent basic dyes, of which, the Alcian blue 8G has been historically the most common and the most reliable member. It is used to stain acidic polysaccharides such as glycosaminoglycans in cartilages and other body structures, some types of mucopolysaccharides, sialylated glycocalyx of cells etc. Alcian blue if often used to identify Chondrocytes from differentiated cultures of both human and rodent mesenchymal stem cells.

Application

Research Category
Stem Cell Research
Research Sub Category
Osteobiology

Arthritis

Physical form

Product is presented as a 1% w/v Alcian Blue solution in acetic acid at pH 2.5.
Product is presented as a 1% w/v Alcian Blue solution.

Preparation Note

Store at room temperature for up to 4 months from date of receipt. If Alcian-Blue solution forms a precipitate, remove particulates by passage through a 0.22 or 0.45-micron filter.

Analysis Note

Solution is blue with no particulates present. Solution is suitable for endpoint cell culture assays.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Was ist los?

WGK 2

Piktogramme

FlameCorrosion
GHS02,GHS05

Signalwort

Danger

Gefahrencodes

H226,H314

Hazard Classifications

Eye Dam. 1 - Flam. Liq. 3 - Skin Corr. 1A

Lagerklasse

3 - Flammable liquids

Flammpunkt (°F)

102.2 °F - closed cup

Flammpunkt (°C)

39 °C - closed cup

Zulassungslistungen

Zulassungslistungen werden hauptsächlich für chemische Produkte erstellt. Für nicht-chemische Produkte können hier nur begrenzte Angaben gemacht werden. Kein Eintrag bedeutet, dass keine der Komponenten gelistet ist. Es liegt in der Verantwortung des Benutzers, die sichere und legale Verwendung des Produkts zu gewährleisten.

64-19-7

CAS No.

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Analysenzertifikate (COA)

Lot/Batch Number

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Questions

1–4 of 4 Questions  

  1. What is the composition of the differentiation for TMS 010 C procedure?

    1 answer

    1. Product TMS-010 is a standard pH 2.5 solution of Alcian Blue that stains a number of substances, including chondrocytes, at this pH. The provided procedure is similar but not identical to the standard histology stain procedure found in most histology textbooks.

      Helpful?

  2. Is there a procedure available for using TMS-010, and in this context, would you anticipate the alcian blue to be present in the nuclei, cytoplasm, or the entire cell?

    1 answer

    1. Depending on the preparation of Alcian Blue, the dye can be used to identify various substances. TMS-010 seems to have been formulated to stain chondrocytes, the cells that produce collagen. It's expected that the staining should be primarily in the cytoplasm. However, if the staining intensity is high, it might appear that the entire cell is stained.

      Helpful?

  3. What is the concentration of acetic acid specified for the Alcian Blue pH 2.5 TMS-010-C product?

    1 answer

    1. The concentration of acetic acid specified for the Alcian Blue pH 2.5 TMS-010-C product is not provided. However, it is a classical textbook formulation typically consisting of 1% Alcian Blue in 3% glacial acetic acid.

      Helpful?

  4. Can you provide a protocol for a colorimetric assay that uses Alcian Blue to compare sulfated proteoglycan content in adherent cancer cell lines?

    1 answer

    1. The Alcian-Blue Staining Solution (cat. no. TMS-010-C) is a cell culture product from the cell culture portfolio, intended for cell-based assays, cell differentiation, and stem cell culture. For example, to identify chondrocytes from differentiated cultures of both human and rodent mesenchymal stem cells (MSCs).

      This product is presented as a 1% w/v Alcian Blue solution in acetic acid at pH 2.5.

      The Certificate of Analysis does not include any usage instructions and there is no separate product data sheet or user guide.

      The following paper may be helpful for the staining of stem cells:
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6432525/

      Helpful?

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