This stock solution may be diluted with sterile water, medium, or an aqueous buffer such as HEPES or phosphate-buffered saline. Sterile saline is also acceptable for dilution.
Fortfahren mit
I0516
Insulin -Lösung aus Rinderpankreas
10 mg/mL insulin in 25 mM HEPES, pH 8.2, BioReagent, sterile-filtered, suitable for cell culture
Synonym(e):
Bovine insulin, Cell culture grade insulin
Anmelden zur Ansicht der Organisations- und Vertragspreise.
Größe auswählen
Ansicht ändern
| Größe/SKU | Verfügbarkeit | Preis |
|---|
Über diesen Artikel
Biological source:
bovine
Form:
solution
Preise und Verfügbarkeit sind derzeit nicht verfügbar.
Technischer Dienst
Benötigen Sie Hilfe? Unser Team von erfahrenen Wissenschaftlern ist für Sie da.
Unterstützung erhaltenbiological source
bovine
Quality Segment
sterility
sterile-filtered
product line
BioReagent
form
solution
concentration
10 mg/mL insulin in 25 mM HEPES, pH 8.2
technique(s)
cell culture | mammalian: suitable, single cell analysis: suitable
impurities
endotoxin, tested
pH
7.9-8.5
UniProt accession no.
shipped in
ambient
storage temp.
2-8°C
SMILES string
SC[C@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)CN)[C@H](CC)C)C(C)C)CCC(=O)O)CCC(=O)N)CS)C(=O)N[C@@H](C)C(=O)N[C@@H](CO)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CS)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](Cc4ccc(cc4)O)C(=O)N[
InChI
1S/2C97H151N25O34S4/c2*1-12-46(10)77(120-72(131)35-98)96(154)122-76(45(8)9)94(152)107-55(24-28-74(134)135)79(137)104-53(22-26-69(100)128)82(140)116-66(40-159)93(151)118-64(38-157)90(148)103-47(11)78(136)114-63(37-124)89(147)121-75(44(6)7)95(153)119-67(41-160)92(150)115-62(36-123)88(146)109-57(30-43(4)5)84(142)110-58(31-48-13-17-50(125)18-14-48)85(143)105-52(21-25-68(99)127)80(138)108-56(29-42(2)3)83(141)106-54(23-27-73(132)133)81(139)112-60(33-70(101)129)87(145)111-59(32-49-15-19-51(126)20-16-49)86(144)117-65(39-158)91(149)113-61(97(155)156)34-71(102)130/h2*13-20,42-47,52-67,75-77,123-126,157-160H,12,21-41,98H2,1-11H3,(H2,99,127)(H2,100,128)(H2,101,129)(H2,102,130)(H,103,148)(H,104,137)(H,105,143)(H,106,141)(H,107,152)(H,108,138)(H,109,146)(H,110,142)(H,111,145)(H,112,139)(H,113,149)(H,114,136)(H,115,150)(H,116,140)(H,117,144)(H,118,151)(H,119,153)(H,120,131)(H,121,147)(H,122,154)(H,132,133)(H,134,135)(H,155,156)/t2*46-,47-,52-,53-,54-,55-,56-,57-,58-,59-,60-,61-,62-,63-,64-,65-,66-,67-,75-,76-,77-/m00/s1
InChI key
XYPANFGXFLOTDK-CJLNJPJHSA-N
Gene Information
bovine ... INS(280829)
cow ... INS(280829)
General description
Application
Insulinlösung aus Rinderpankreas wurde wie folgt verwendet:
- zur Untersuchung der Wirkung von exogenem Insulin auf die Plasmakonzentrationen von Glucose, Insulin, Insulin-like growth factor I (IGF-I) und Progesteron (P4) in pubertären Jungrindern
- zur Untersuchung der Insulinreaktion der Plazenta bei Schwangerschaftskomplikationen mit primären humanen Trophoblastenzellen und villösen Plazentaexplantaten
- zur Untersuchung der Auswirkung der Dichte der dendritischen Dornen bei der Insulinrezeptorsignalisierung in Hippocampus-Neuronen von neugeborenen Mäusen in vitro
Biochem/physiol Actions
Insulin ist ein wichtiges glucosesenkendes Hormon, dessen Insuffizienz zu Hyperglykämie führt, wie sie bei Typ-II-Diabetes beobachtet wird.
Zweikettige Polypeptidhormone die durch die β-Zellen der Langerhans-Inseln produziert werden. Ihr Molekulargewicht ist ~5800 Da. Die α- und β-Ketten sind durch zwei Disulfidbrücken verbunden. Die α−Kette enthält eine intrakettige Disulfidbindung. Insulin reguliert die zelluläre Aufnahme, Nutzung und Speicherung von Glucose, Aminosäuren und Fettsäuren und hemmt den Abbau von Glykogen, Eiweiß und Fett.
Physical form
Stabil bei 2 bis 8 °C
1 of 1
Dieser Artikel | |||
|---|---|---|---|
| description 10 mg/mL insulin in 25 mM HEPES, pH 8.2, BioReagent, sterile-filtered, suitable for cell culture | description ≥80% (HPLC), powder | description powder, ≥27 USP units/mg | description ≥80% (HPLC), powder |
| biological source bovine | biological source bovine | biological source Porcine pancreas | biological source bovine |
| product line BioReagent | product line - | product line - | product line - |
| Quality Level 400 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| technique(s) cell culture | mammalian: suitable, single cell analysis: suitable | technique(s) - | technique(s) cell culture | mammalian: suitable | technique(s) - |
| concentration 10 mg/mL insulin in 25 mM HEPES, pH 8.2 | concentration - | concentration - | concentration - |
| form solution | form powder | form powder | form powder |
Lagerklasse
12 - Non Combustible Liquids
PSA (Persönliche Schutzausrüstung)
Eyeshields, Gloves
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Hier finden Sie alle aktuellen Versionen:
Besitzen Sie dieses Produkt bereits?
In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
-
Is there a specific solution that I should use to further dilute or is sterile saline ok?
1 answer-
Helpful?
-
-
Can I freeze the aliquots at -20 or -80 for the long-term storage?
1 answer-
The USP recommends that solutions of insulin be stored at 2-8°C. It is not recommended to freeze solutions of insulin, as it can aggregate. Sterile solutions should be stable for at least 1 year at 2-8°C.
Helpful?
-
-
What is the shelf life of this product if stored at 2-8°C ? Thanks.
1 answer-
This product is assigned an expiration date of 2 years from the date of manufacture. The expiration date is lot-specific and is printed on the label, as well as in the Certificate of Analysis. Please navigate to the ‘DOCUMENTATION’ section of the Product Detail Page to access a Certificate under ‘Certificate of Analysis’:
https://www.sigmaaldrich.com/product/sigma/i0516#product-documentationHelpful?
-
-
Is bovine insulin (I0516) suitable for use in rat cell culture?
1 answer-
The product has not been validated for rat cell culture application. However, based on the following citations, it appears to work well with rat cell culture:
•Isolation, Transfection, and Long-Term Culture of Adult Mouse and Rat Cardiomyocytes
published in J Vis Exp. 2020 Oct 10;(164):10.3791/61073. PMID: 33104067.•Collagen hydrogels with controllable combined cues of elasticity and topography to regulate cellular processes" published in Biomed Mater. 2021 Jun 11;16(4). PMID: 34030146.
Helpful?
-
-
Does this solution contain zinc? How would that affect how quickly the insulin would act in a mammalian cell culture?
1 answer-
The product contains 10 mg/mL insulin in 25 mM HEPES, pH 8.2, but it does not include zinc. Currently, the effect of zinc on insulin signaling is not characterized. However, it is noted that zinc appears to be able to enhance insulin-mediated IR tyrosine phosphorylation according to the article Biol Trace Elem Res. 2004 Oct;101(1):19-36. Effect of chromium and zinc on insulin signaling in skeletal muscle cells. PMID: 15516700.
Helpful?
-
-
is this product suitable for 3T3-L1 differentiation?
1 answer-
This product is supplied as a sterile bovine insulin solution at 10 mg/mL and would be suitable for diffentiation assays. This item has not been tested on 3T3-L1 cells, but is part of the differentiation protocol for the WT-1 preadipocyte cell line, product SCC255. See the link below to review this protocol:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/568/334/scc255ds.pdfHelpful?
-
-
What is the molarity of Product I0516, Insulin solution?
1 answer-
Product No. I0516 is a 10 mg/mL solution of bovine insulin (MW 5733.5). The molarity is 1.7 μmol/mL or 1.7 mM.
Helpful?
-
-
What is the amino acid sequence for insulin?
1 answer-
The sequence and structure of insulin can be found on the product information sheet (under Documents, above).
Helpful?
-
-
What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
-
-
How many units/mg am I getting in Product I0516, Insulin solution?
1 answer-
This solution is made using insulin Product No. I5500. The potency specification for the I5500 is not less than 27 USP units/mg. However, we do not test for potency of the final product of this solution. It is possible some activity is lost during the manufacturing process.
Helpful?
-